[Chemical Constituents from Cynomorium songaricum].

Objective: To study the chemical constituents from the fleshy stems of Cynomorium songaricum. Methods: The chemical constituents were isolated and purified by chromatography on MCI, silica gel, Sephadex LH-20 columns, etc. The structures of compounds were elucidated by physicochemical property and spectral analyses. Results: 15 compounds were isolated and identified as choerospondin( 1), isolariciresinol-4-O-ß-D-glucopyranoside( 2), lutelin-7-O-ß-D-glucopyranoside( 3), 3, 4-dihydroxyphenethylacetate( 4),maslinic acid( 5),catechin ( 6),ursolic acid( 7),gentisic acid( 8),mannitol( 9), phloroglucinol( 10), ß-sitosterol( 11), daucosterol( 12), rutin( 13), cetylic acid( 14) and epicatechin( 15). Conclusion: Compounds 4,5,8,9 and 10 are obtained from this plant for the first time.

Preparation of molecularly imprinted polymers based on magnetic nanoparticles for the selective extraction of protocatechuic acid from plant extracts.

In this study, highly selective core-shell molecularly imprinted polymers on the surface of magnetic nanoparticles were prepared using protocatechuic acid as the template molecule. The resulting magnetic molecularly imprinted polymers were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometry. The binding performances of the prepared materials were evaluated by static and selective adsorption. The binding isotherms were obtained for protocatechuic acid and fitted by the Langmuir isotherm model and Freundlich isotherm model. Furthermore, the resulting materials were used as the solid-phase extraction materials coupled to high-performance liquid chromatography for the selective extraction and detection of protocatechuic acid from the extracts of Homalomena occulta and Cynomorium songaricum with the recoveries in the range 86.3-102.2%.