RESUMO
An aflatoxin B1 metabolite was found to become covalently bound to rat liver RNA and calf thymus DNA in vitro, and it formed complexes with increased spectral absorbance in the 360 nm region. The formation of such complexes was reduced nicotinamide adenine dinucleotide phosphate and microsome dependent, was inhibited by theta-diethylaminoethyl diphenylpropylacetate-HC1, and by CO and N2, when the latter were used to replace the gas phase of the incubations. The formation of the complexes was enhanced about 2-fold with cicrosomes from phenobarbital-treated rats but not from 3-methylcholanthrene-treated rats. More binding was observed with DNA than RNA. Dentured DNA was about 70% as effective as native DNA. Nucleic acids from various sources showed the following order of binding potency: DNA from Micrococcus luteus greater than DNA from calf thymus equal to DNA from rat liver greater than RNA from rat liver greater than transfer RNA from rat liver. In the presence of reduced nicotinamide adenine dinucleotide phosphate and microsomes from phenobarbital-treated rats, aflatoxin G1 was also converted into metabolite(s) that became covalently bound to nucleic acids and formed complexes with increased spectral absorbances in the 360 nm region: this reaction was also inhibited by theta-diethylaminoethyl diphenylpropylacetate-HC1. Under the same conditions, aflatoxin B2, aflatoxin G2, aflatoxin B2a, and "Compound 11," which lack a C2-C3 double bond, did not show any noticeable binding to either DNA or RNA. These data strongly support the concept that the microsomal mixed-funciton oxygenase-catalyzed oxidation of the C2-C3 double bond of aflatoxins is a prerequisite for the formation of nucleic acid-binding metabolites. Microsomes from untreated, phenobarbital-treated, and 3-methylcholanthrene-treated rats were compared in vitro for their ability to catalyze the formation of DNA-binding metabolites from aflatozin B1 and benzo(a)pyrene. In assays involving benzo(a)pyrene, microsomes from 3-methylcholanthrene-treated rats were 12- and 5-fold more active than microsomes from untreated and phenobar-bital-treated rats, respectively. This is in contrast to the results obtained with aflatoxin B1 and suggests that different enzymes in the hepatic microsomal mixed-function oxygenase complex are involved in the generation of reactive metabolites from various polycyclic hydrocarbons.
Assuntos
Aflatoxinas/metabolismo , Benzopirenos/metabolismo , DNA/metabolismo , RNA/metabolismo , Animais , Monóxido de Carbono , Fracionamento Celular , Fígado/metabolismo , Metilcolantreno/farmacologia , Micrococcus/metabolismo , Microssomos Hepáticos/enzimologia , NADP/farmacologia , Nitrogênio , Desnaturação de Ácido Nucleico , Fenobarbital/farmacologia , Proadifeno/farmacologia , RNA de Transferência/metabolismo , Ratos , Análise Espectral , Timo/metabolismo , TrítioRESUMO
Administration of SKF-525A to rats fed on a stock diet specifically decreased the serum concentration of low-density lipoprotein. SKF-525A and cholestryamine also reversed the rise in circulating concentration of both very-low density and low-density lipoprotein that was observed in rats given a sucrose-based, cholesterol-supplemented diet. The enhancement of hepatic cholesterol 7 alpha-hydroxylase by SKF-525A or by cholestyramine is accompanied by homeostatic responses by the liver which include induction of low-density lipoprotein clearance and increased cholesterogenesis to attempt to replenish sterol pools. These compensatory mechanisms are separately controlled.
Assuntos
Colesterol 7-alfa-Hidroxilase/metabolismo , Hiperlipoproteinemias/enzimologia , Lipoproteínas LDL/sangue , Proadifeno/farmacologia , Esteroide Hidroxilases/metabolismo , Animais , Colesterol/metabolismo , Resina de Colestiramina/farmacologia , Dieta , Interações Medicamentosas , Hiperlipoproteinemias/tratamento farmacológico , Lipídeos/sangue , Lipoproteínas/sangue , Masculino , Ratos , Ratos EndogâmicosRESUMO
Oral administration of O,O,S-trimethyl phosphorothioate (OOS), an impurity present in widely used organophosphorus insecticides, causes delayed toxicity in rats, i.e., death occurring as late as 28 days after the treatment. The signs of toxicity include body weight loss (maximum on day 3), red staining around the nose, mouth and eyes, and an increased level of lactate dehydrogenase (LDH) in bronchopulmonary lavage fluid accompanied by morphological alteration of non-ciliated bronchiolar epithelial Clara cells. Pretreatment with phenobarbital, piperonyl butoxide (2 h), SKF 525-A, or small multiple doses of OOS protected against the OOS-induced elevated level of bronchopulmonary lavage LDH, and the other signs of delayed toxicity including morphological alteration of Clara cells. These studies support the view that OOS-induced delayed toxicity is mediated by the cytochrome P-450 dependent metabolism of OOS, and the lung may be the major target organ of delayed toxicity produced by OOS.
Assuntos
Organotiofosfatos/toxicidade , Compostos Organotiofosforados/toxicidade , Fenobarbital/farmacologia , Butóxido de Piperonila/farmacologia , Proadifeno/farmacologia , Animais , Peso Corporal/efeitos dos fármacos , L-Lactato Desidrogenase/metabolismo , Dose Letal Mediana , Pulmão/efeitos dos fármacos , Pulmão/enzimologia , Masculino , Organotiofosfatos/administração & dosagem , Ratos , Ratos EndogâmicosRESUMO
In vitro rates of deacetylation were measured with carcinogenic and noncarcinogenic isomeric arylamides using microsomal preparations from guinea pig, hamster, rat, mouse, rabbit and dog. Following incubation at 37 degrees, the substrate and hydrolysis products were extracted into ether and the corresponding aromatic amine quantified by gas-liquid chromatography. The data show that rates of deacetylation were both species and isomer dependent. In general, the highest rate of deacetylation in microsomes from all species was recorded with 2-acetamidobiphenyl (2-AABP); however, the mouse uniquely deacetylated 4-acetamidobiphenyl (4-AABP) most rapidly. The meta isomer 3-acetamidobiphenyl (3-AABP) was deacetylated at an intermediate rate in all species investigated. The variability in the rates of deacetylation within species may reflect the heterogeneity of the deacetylase enzymes and may be an important factor in amine/amide carcinogenesis.
Assuntos
Compostos de Aminobifenil/metabolismo , Microssomos Hepáticos/metabolismo , Animais , Cricetinae , Cães , Feminino , Cobaias , Isomerismo , Masculino , Mesocricetus , Camundongos , Camundongos Endogâmicos , Microssomos Hepáticos/efeitos dos fármacos , Paraoxon/farmacologia , Proadifeno/farmacologia , Coelhos , Ratos , Ratos Endogâmicos , Fluoreto de Sódio/farmacologia , Especificidade da EspécieRESUMO
The histamine type-2 receptor antagonist and cytochrome P450 inhibitor cimetidine was examined for its antimalarial properties in the presence or absence of chloroquine or pyrimethamine. When used alone, cimetidine displayed little activity against a number of Plasmodium falciparum clones in vitro, with an IC50 of 300-700 microM. The compound was found to be highly synergistic in combination with chloroquine and also displayed a degree of synergism when used in combination with pyrimethamine. These synergistic effects were independent of the chloroquine- or pyrimethamine-resistance status of the clones. The cytochrome P450 inhibitor proadifen displayed weak synergism or antagonism with chloroquine, depending on the clone tested, and clear antagonism with pyrimethamine. The results with proadifen indicate that cimetidine was exerting its synergistic activity via a mechanism distinct from inhibition of cytochrome P450. Additional experiments have demonstrated that cimetidine interferes with neither plasmodial sterol metabolism nor heme polymerization.
Assuntos
Antimaláricos/farmacologia , Cloroquina/farmacologia , Cimetidina/farmacologia , Antagonistas dos Receptores H2 da Histamina/farmacologia , Plasmodium falciparum/efeitos dos fármacos , Pirimetamina/farmacologia , Animais , Inibidores das Enzimas do Citocromo P-450 , Resistência a Medicamentos , Sinergismo Farmacológico , Inibidores Enzimáticos/farmacologia , Heme/antagonistas & inibidores , Heme/química , Plasmodium falciparum/enzimologia , Polímeros , Proadifeno/farmacologiaAssuntos
Aciltransferases/metabolismo , Artérias/ultraestrutura , Colesterol/metabolismo , Frações Subcelulares/metabolismo , Aciltransferases/antagonistas & inibidores , Trifosfato de Adenosina/farmacologia , Animais , Cromatografia em Camada Fina , Coenzima A , Depressão Química , Dextranos/farmacologia , Ésteres , Etilaminas/farmacologia , Heparina/farmacologia , Concentração de Íons de Hidrogênio , Técnicas In Vitro , Masculino , Microssomos/efeitos dos fármacos , Microssomos/metabolismo , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Ácidos Palmíticos/metabolismo , Éteres Fenílicos/farmacologia , Fosfato de Polifloretina/farmacologia , Polissorbatos/farmacologia , Proadifeno/farmacologia , Suínos , Ácido Taurocólico/farmacologiaAssuntos
Microssomos Hepáticos/enzimologia , Oxigenases de Função Mista/metabolismo , Oxirredutases/metabolismo , Rifampina/farmacologia , Animais , Benzoflavonas/farmacologia , Sistema Enzimático do Citocromo P-450/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microssomos Hepáticos/efeitos dos fármacos , NADPH-Ferri-Hemoproteína Redutase/metabolismo , Fenobarbital/farmacologia , Carbonitrila de Pregnenolona/farmacologia , Proadifeno/farmacologia , Fluoreto de Sódio/farmacologia , beta-NaftoflavonaAssuntos
Hiperplasia Gengival/induzido quimicamente , Fenitoína/sangue , Animais , Cromatografia Gasosa , Colorimetria , Feminino , Gengiva/patologia , Hiperplasia Gengival/patologia , Meia-Vida , Masculino , Metabolismo/efeitos dos fármacos , Fenitoína/metabolismo , Fenitoína/toxicidade , Proadifeno/farmacologia , Ratos , Especificidade da EspécieAssuntos
Bromobenzenos/toxicidade , Fígado/efeitos dos fármacos , Animais , Biopolímeros , Biotransformação , Bromobenzenos/metabolismo , Fígado/metabolismo , Metilcolantreno/farmacologia , Microssomos Hepáticos/efeitos dos fármacos , Microssomos Hepáticos/metabolismo , Fenobarbital/farmacologia , Proadifeno/farmacologia , RatosRESUMO
A rapid, sensitive, and simple high-performance liquid chromatography method is described for the direct analysis of aminopyrine in saliva. The detection limit was found to be 12 ng/ml of sample. Accuracy and precision were maintained with as little as 1 microliter of saliva. Thus the rate of elimination of aminopyrine has been monitored noninvasively in rats. The aminopyrine half-life was found to be 59.5 +/- 10.5 (SD) min. The half-life was independent of an aminopyrine dose between 50 and 200 mg/kg. In rats single intraperitoneal doses of SKF 525-A (20 mg/kg) produced increases in aminopyrine half-life and salivary aminopyrine concentration. Phenobarbital induction lowered the aminopyrine half-life to 33.2 +/- 8.5 min and the salivary aminopyrine concentration.
Assuntos
Aminopirina/metabolismo , Fígado/metabolismo , Animais , Meia-Vida , Masculino , Proadifeno/farmacologia , Ratos , Ratos Endogâmicos , Saliva/metabolismoRESUMO
1. Medullipin I (Med I) is a hormone extracted from renal papillae and its renomedullary interstitial cells (RIC). Med I is stimulated by elevation of the renal artery perfusion pressure. 2. When isolated normal rat kidneys were perfused either with oxygenated blood or with 5% albumin bubbled with O2 at elevated perfusion pressures, Med I appeared to be secreted into the renal venous effluent (RVE). Addition of Tween 20, treatment of the assay rat with SKF 525A, inhibitor of cytochrome P-450 and removal of the liver from the systemic circulation prevented vasodepression of both the RVE and extracted Med I. The lipid in the RVE gave the same dose-response as extracted Med I. 3. Lowering the renal artery perfusion pressure below normal inhibited the secretion of Med I. As the perfusion pressure was elevated Med I secretion appeared to increase. 4. Previous observations and the present study support the view that the renin-angiotensin system and the Medullipin system are double feedback systems involved in blood pressure control.
Assuntos
Hipertensão Renovascular/fisiopatologia , Rim/metabolismo , Metabolismo dos Lipídeos , Angiotensina II/biossíntese , Animais , Pressão Sanguínea/fisiologia , Técnicas In Vitro , Rim/irrigação sanguínea , Leucotrienos/biossíntese , Lipídeos , Masculino , Perfusão , Polissorbatos/farmacologia , Proadifeno/farmacologia , Prostaglandinas/biossíntese , Ratos , Ratos Endogâmicos , Artéria Renal/fisiologiaRESUMO
A new syndrome is described in a patient with advanced renal insufficiency. This consists of severe and persistent hypotension causing weakness but associated with a clear mental status. Also present is evidence for decreased vascular reactivity. The hypotension was not orthostatic. The hypotension was associated with a circulating vasodepressor substance having the characteristics of medullipin 1. The medullipin appears to have been derived from the remaining right kidney. Hypotension existed despite the presence of major prohypertensive mechanisms, including an endstage kidney, hyperreninemia and hyperaldosteronemia. It is likely that hypotension due to hypermedullipinemia is an entity occurring in the human being.
Assuntos
Anti-Hipertensivos/sangue , Hipotensão/etiologia , Medula Renal/fisiologia , Lipídeos/sangue , Adulto , Animais , Pressão Sanguínea , Feminino , Humanos , Hipotensão/sangue , Polissorbatos/farmacologia , Proadifeno/farmacologia , Ratos , Ratos Endogâmicos SHR , SíndromeRESUMO
The glucuronide of 7-hydroxychlorpromazine (CPOH), formed by incubation with hepatic microsomes and UDP-[U-14C]glucuronic acid, was isolated by extraction into 1-butanol at pH 1 and assayed by liquid scintillation counting. Although concentrations of CPOH greater than 3 X 10(-4) M stimulated its conjugation, the apparent KM of the transferase in microsomes from guinea pig liver for CPOH was 9.5 X 10(-5) M. The apparent activation of glucuronyltransferase by high concentrations of CPOH was prevented when preparations were maximmally stimulated by Triton X-100. The rate of conjugation of CPOH by preparations of guinea pig liver was almost twice that measured with those of rat liver. However, the maximal stimulation of the conjugation of CPOH by Triton with preparations of rat liver was almost twice that observed with microsomes from guinea pig liver. The conjugation of CPOH by microsomes from rat and guinea pig liver was inhibited about 25% by SKF 525-A (1 X 10(-4) M). The inhibitory action of SKF 525-A decreased at concentrations greater than 5 X 10(-4) M and no inhibition was observed at 1 X 10(-3) M.
Assuntos
Clorpromazina/análogos & derivados , Animais , Clorpromazina/metabolismo , Cromatografia em Papel , Estradiol/metabolismo , Estrona/metabolismo , Glucuronatos/biossíntese , Cobaias , Concentração de Íons de Hidrogênio , Hidroxilação , Técnicas In Vitro , Masculino , Microssomos Hepáticos/efeitos dos fármacos , Microssomos Hepáticos/metabolismo , Polietilenoglicóis/farmacologia , Proadifeno/farmacologia , Ratos , Relação Estrutura-Atividade , Fatores de TempoRESUMO
This study focused on the mechanisms of the negative inotropic response to bradykinin (BK) in isolated rat hearts perfused at constant flow. BK (100 nM) significantly reduced developed left ventricular pressure (LVP) and the maximal derivative of systolic LVP by 20-22%. The cytochrome P-450 (CYP) inhibitors 1-aminobenzotriazole (1 mM and 100 microM) or proadifen (5 microM) abolished the cardiodepression by BK, which was not affected by nitric oxide and cyclooxygenase inhibitors (35 microM NG-nitro-L-arginine methyl ester and 10 microM indomethacin, respectively). The CYP metabolite 14,15-epoxyeicosatrienoic acid (14,15-EET; 50 ng/ml) produced effects similar to those of BK in terms of the reduction in contractility. After the coronary endothelium was made dysfunctional by Triton X-100 (0.5 microl), the BK-induced negative inotropic effect was completely abolished, whereas the 14,15-EET-induced cardiodepression was not affected. In hearts with normal endothelium, after recovery from 14,15-EET effects, BK reduced developed LVP to a 35% greater extent than BK in the control. In conclusion, CYP inhibition or endothelial dysfunction prevents BK from causing cardiodepression, suggesting that, in the rat heart, endothelial CYP products mediate the negative inotropic effect of BK. One of these mediators appears to be 14,15-EET.