Detection and quantification of MUC7 in submandibular, sublingual, palatine, and labial saliva by anti-peptide antiserum.
J Dent Res
; 78(7): 1362-9, 1999 Jul.
Article
en En
| MEDLINE
| ID: mdl-10403464
ABSTRACT
The large carbohydrate moiety of low-Mr salivary mucin MUC7 (originally referred to as MG2) is subject to variations. Biochemical analysis and quantification of MUC7 in saliva samples require recognition tools that are independent of the carbohydrate moiety. Therefore, we have evoked three antisera to synthetic peptides of MUC7. One of these (CpMG2), raised against the C-terminal peptide, recognized native MUC7 in saliva and was characterized further. Recognition of MUC7 by CpMG2 turned out to be specific, resistant to dissociating and reductive treatments, and independent of glycosylation differences, as indicated by Western analysis and ELISA. The antiserum could be used to monitor MUC7 during purification procedures. MUC7 was demonstrated in small volumes of saliva from all (sero)mucous glands, including the palate and lip. Analysis with antibodies and lectins indicated large variations in amount as well as in glycosylation of MUC7. An ELISA was developed to determine the relative quantity of MUC7 in the glandular salivas mean values of approximately 220, 980, and 100 microg mucin per mL were found in submandibular, sublingual, and palatine saliva, respectively.
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Banco de datos:
MEDLINE
Asunto principal:
Saliva
/
Glándulas Salivales Menores
/
Proteínas y Péptidos Salivales
/
Glándula Sublingual
/
Glándula Submandibular
/
Mucinas
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Dent Res
Año:
1999
Tipo del documento:
Article
País de afiliación:
Países Bajos