[Effect of surfactants on the in vitro and in vivo properties of amphotericin B liposome].
Yao Xue Xue Bao
; 38(6): 471-4, 2003 Jun.
Article
en Zh
| MEDLINE
| ID: mdl-14513812
ABSTRACT
AIM:
Some surfactants such as DSPE-PEG, Tween 80 and Brij 35 were used to modify the amphotericin B liposome, improve the stability, optimize the tissue distribution and decrease the toxicity of amphotericin B liposome.METHODS:
The amphotericin B liposome was prepared by the film-supersound method. The effects of cholesterol and amphotericin B on the encapsulation percentage were studied. The diameter, leakage percentage in phosphate buffer solution(PBS) and calf blood serum, and tissue distributions of amphotericin B liposome in the rat were determined.RESULTS:
The top encapsulation percentage of amphotericin B liposome is (91.2 +/- 1.6)%. After modification with DSPE-PEG, Tween 80 and Brij 35, the encapsulation percentages were improved, the average diameters were decreased and the stabilities were improved, the amphotericin B concentrations in the liver, spleen and kidney were decreased, and the amphotericin B concentrations in the brain were increased, especially in the AmB-L-Tween 80 group.CONCLUSION:
DSPE-PEG and Brij 35 could decrease the clearing of reticuroendothelial systems(RES) to the amphotericin B liposome and Tween 80 could facilitate the transporting of amphotericin B liposome into the brain.
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Banco de datos:
MEDLINE
Asunto principal:
Tensoactivos
/
Anfotericina B
/
Antifúngicos
Límite:
Animals
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Año:
2003
Tipo del documento:
Article
País de afiliación:
China