[Bone defect repair with a new tissue-engineered bone carrying bone morphogenetic protein in rabbits].
Di Yi Jun Yi Da Xue Xue Bao
; 25(11): 1369-74, 2005 Nov.
Article
en Zh
| MEDLINE
| ID: mdl-16305958
ABSTRACT
OBJECTIVE:
To construct a new tissue-engineered bone with poly (D, L-lactide-co-glycolide) (PLGA), bone morphogenetic protein (BMP) and bone marrow-derived stem cells (BMSCs) and observe its effect in repairing segmental bone defects.METHODS:
A 15-mm bone defect in the right radius was induced in New Zealand white rabbits, and the models were randomized into three groups to receive implantation of the tissue-engineered bone grafts constructed with PLGA carrying 5 mg BMP and about 1 x 10(6) BMSCs (experimental group), grafts of PLGA with about 1 x 10(6) BMSCs (control group), or grafts of exclusive PLGA (blank control group), respectively. The osteogenesis in the bone defect after the implantation on was evaluated X-ray films, and the histological changes of the tissues sampled from the bone defect 4, 8, and 12 weeks after operation were observed and new bone formation was measured by image analysis.RESULTS:
The bone defect was completely repaired in the experimental group 12 weeks after the implantation, showing the best results among the 3 groups. The bone defects in the blank control group was filled with only fibrous and connective tissues at 12 weeks.CONCLUSION:
This tissue-engineered bone constructed with PLGA, BMP and BMSCs possesses good ability in repairing segmental bone defect.
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Banco de datos:
MEDLINE
Asunto principal:
Ácido Poliglicólico
/
Polímeros
/
Fracturas del Radio
/
Ácido Láctico
/
Proteínas Morfogenéticas Óseas
/
Ingeniería de Tejidos
/
Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
Zh
Revista:
Di Yi Jun Yi Da Xue Xue Bao
Asunto de la revista:
MEDICINA
Año:
2005
Tipo del documento:
Article
País de afiliación:
China