[Production of ligninolytic enzymes in bioreactor].
Huan Jing Ke Xue
; 27(2): 333-7, 2006 Feb.
Article
en Zh
| MEDLINE
| ID: mdl-16686200
ABSTRACT
Production of ligninolytic enzymes under nitrogen limited conditions(C/N = 56/2.2) was studied in a 5-L stirred tank bioreactor with a working volume of 2 L for obtaining higher production of ligninolytic enzymes by white rot fungus Phanerochaete chrysosporium BKM-F-1767 and its control strategy. Results show that the manganese peroxidase (MnP) and laccase (Lac) reached peak at the sixth day and the seventh day, respectively, and the variation of them with time in a batch cultivation are similar to the results by agitated Erlenmeyer flasks; however higher enzyme activity was not achieved by applying a fed-batch strategy, in which nitrogen limited medium was fed to the reactor. In addition, variation of pH during cultivation was related to the growth of P. chrysosporium and enzymes production during both batch and fed-batch cultivation. The pH value of liquid medium began to decline when the enzyme activity occurred in the system, and the decline became more and more slow along with the decrease of enzyme activity at the end of fermentation. So, pH would be as a control parameter to find out the growth of P. chrysosporium and enzymes production during incubating P. chrysosporium. However, fed-batch strategy still need further study.
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Banco de datos:
MEDLINE
Asunto principal:
Peroxidasas
/
Reactores Biológicos
/
Phanerochaete
/
Lacasa
Idioma:
Zh
Revista:
Huan Jing Ke Xue
Año:
2006
Tipo del documento:
Article
País de afiliación:
China