Development of a diffraction imaging flow cytometer.
Opt Lett
; 34(19): 2985-7, 2009 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-19794790
ABSTRACT
Diffraction images record angle-resolved distribution of scattered light from a particle excited by coherent light and can correlate highly with the 3D morphology of a particle. We present a jet-in-fluid design of flow chamber for acquisition of clear diffraction images in a laminar flow. Diffraction images of polystyrene spheres of different diameters were acquired and found to correlate highly with the calculated ones based on the Mie theory. Fast Fourier transform analysis indicated that the measured images can be used to extract sphere diameter values. These results demonstrate the significant potentials of high-throughput diffraction imaging flow cytometry for extracting 3D morphological features of cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Óptica y Fotónica
/
Citometría de Flujo
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Opt Lett
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos