The upregulation of tumour necrosis factor-α and surface antigens expression on macrophages by bisphenol A-glycidyl-methacrylate.
Int Endod J
; 45(7): 619-26, 2012 Jul.
Article
en En
| MEDLINE
| ID: mdl-22268514
ABSTRACT
AIM:
To evaluate the expression of tumour necrosis factor-α and surface antigens by bisphenol A-glycidyl-methacrylate (BisGMA) on murine macrophage cell line RAW264.7.METHODOLOGY:
Cytotoxicity was measured by tetrazolium bromide reduction assay. Tumour necrosis factor (TNF)-α was analysed by enzyme-linked immunosorbent assay. Cell surface antigens were investigated by flowcytometry. Statistical analyses were performed using anova followed by the Bonferroni's t-test for multigroup comparisons.RESULTS:
BisGMA exhibited cytotoxicity to RAW264.7 in a dose-dependent manner (P < 0.05) during 2-h incubation period. BisGMA was found to increase TNF-α secretion in a dose-dependent manner (P < 0.05). In addition, CD11, CD14, CD45, CD54, CD40, CD80, and MHC II were significantly stimulated by BisGMA in a dose-dependent manner (P < 0.05). However, MHC I expression was not affected by BisGMA (P > 0.05).CONCLUSIONS:
Taken together, the ability of macrophages to induce an appropriate immune response when exposed to BisGMA has the potential to upregulate TNF-α production and expression of surface antigens.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Factor de Necrosis Tumoral alfa
/
Bisfenol A Glicidil Metacrilato
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Int Endod J
Año:
2012
Tipo del documento:
Article
País de afiliación:
Taiwán