A mystery wrapped in an enigma: Matrigel enhancement of mammary cell growth and morphogenesis.
J Mammary Gland Biol Neoplasia
; 17(2): 99-101, 2012 Jun.
Article
en En
| MEDLINE
| ID: mdl-22581302
ABSTRACT
The analysis of normal mammary morphogenesis is facilitated by the use of mammary fat pad transplantation. The recent experiments on analysis of normal mammary epithelial stem cell activity rely heavily on this technique. In this review, we discuss the known and unknown attributes of using Matrigel in the injection of the mammary epithelial cell suspension. Matrigel greatly increases the "take" frequency of the injected cell suspension; however, there is some uncertainty regarding the interpretation of some of the results. After consideration of these issues, our conclusion is that Matrigel is important in order to obtain rigorous and reproducible results.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteoglicanos
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Materiales Biocompatibles
/
Colágeno
/
Laminina
/
Células Madre Adultas
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Glándulas Mamarias Animales
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Modelos Biológicos
/
Morfogénesis
Límite:
Animals
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Female
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Humans
Idioma:
En
Revista:
J Mammary Gland Biol Neoplasia
Asunto de la revista:
NEOPLASIAS
Año:
2012
Tipo del documento:
Article
País de afiliación:
Estados Unidos