[A comparative study of the cytotoxicity of five dental bonding agents to human periodontal ligament cells].

ABSTRACT

PURPOSE: To compare the cytotoxicity of 5 different dental bonding agents to human periodontal ligament cells(HPDLCs). METHODS: Primary cultured HPDLCs were exposed to different concentrations (100%,50% and 25%) of Super Bond, Clearfil SE Bond, G-Bond, Single Bond2, and Adper Easy One for different times (24 h, 48 h and 72 h). Cytotoxicity of 5 different agents to HPDLCs were observed by MTT method. The data was analyzed using SPSS 19.0 software package. RESULTS: Cytotoxicity of 5 dentin bonding agents to HPDLCs were Super Bondexposure time and concentration. Cytotoxicity effect was 25%<50%<100% and 24 h<48 h<72 h. Significant difference was found between 24h and 48h(P<0.05) but no significant difference was found between 48h and 72h (P>0.05).

CONCLUSIONS:

Cytotoxicity of 5 dentin bonding agents are different. Cytotoxicity of Super-bond C&B,G-Bond and Clearfil SE Bond are weak, while cytotoxicity of Single Bond2 is the strongest among the 5 agents. Supported by Medical Leading Talents and Innovation Team Project of Jiangsu Province (LJ201110) and Natural Science Foundation of Jiangsu Province (SBK201340904).