Evaluation of the effectiveness of micro-Raman spectroscopy in monitoring the mineral contents change of human enamel in vitro.
Lasers Med Sci
; 32(5): 985-991, 2017 Jul.
Article
en En
| MEDLINE
| ID: mdl-28365815
ABSTRACT
The purpose of this in vitro study was to investigate the efficacy of micro-Raman spectroscopy on detecting mineral content change during the demineralization and de/remineralization cycling process. The enamel samples (n = 55) were randomly divided into three groups and separately treated with demineralization solution (n = 20), de/remineralization cycling solution (n = 30), and distilled water (n = 5). Micro-Raman spectroscopy, microhardness (MHS), and the released calcium ions concentration were performed before and after treatment, respectively. A one-way analysis of variance (ANOVA) with a post hoc Tukey test was used to analyze the results. The Spearman correlation coefficients among the parameters of Raman relative intensity decrease (RRID%), the percentage of MHS loss (PML), and the released calcium ions concentration were also analyzed. In demineralization group, RRID%, PML, and released calcium ions concentration were highly correlated with each other (r = 0.979, p < 0.001; r = 0.984, p < 0.001; and r = 0.983, p < 0.001, respectively). While for the de/remineralization cycling group, there also existed a high correlation between RRID% and PML (r = 0.987, p < 0.001). In conclusion, micro-Raman spectroscopy could effectively monitor the mineral content change, and its efficacy was validated by the measurement of released calcium ions concentration and MHS.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría Raman
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Esmalte Dental
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Minerales
Límite:
Humans
Idioma:
En
Revista:
Lasers Med Sci
Asunto de la revista:
BIOTECNOLOGIA
/
RADIOLOGIA
Año:
2017
Tipo del documento:
Article