Antibacterial and osteoinductive biomacromolecules composite electrospun fiber.
Int J Biol Macromol
; 143: 958-967, 2020 Jan 15.
Article
en En
| MEDLINE
| ID: mdl-31739052
ABSTRACT
Bone implant materials have been widely used in bone therapy. However, bone infections caused by bacteria can damage the healing and repairing ability of bone tissue, which remains a major problem to be solved in clinical bone regeneration with implants. In this study, silver nanoparticles (Ag-NPs) were uniformly distributed on the inner of Polylactic acid and Gelatin composite fibers (PLLA and Gel, biological macromolecules) by co-electrospinning for improving anti-infection ability and osseointegration. The optimized experimental conditions for this method were having PLLA/Gel mass ratio of 9010 and Ag content of 7%. Ag-NPs acted as heterogeneous nucleation sites for promoting the nucleation and growth of calcium phosphorus on the surface of composite fiber. Bone marrow-derived mesenchymal stem cells adhered and proliferated well on the surface of the composite fiber, and the positive fluorescence staining indicated the existence of osteoblasts. Vascular endothelial cells had a good adherence and proliferation on the surface of composite fiber, showing good angiogenic properties. Antibacterial rate of the composite fiber was all over 97% against Monilia albicans, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, showing good antibacterial property. A multi-functional biomacromolecules composite fiber was constructed and shown good bioactivity, osteoinductivity, angiogenic and antibacterial properties.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Osteogénesis
/
Materiales Biocompatibles
/
Sustancias Macromoleculares
/
Nanofibras
/
Antibacterianos
Idioma:
En
Revista:
Int J Biol Macromol
Año:
2020
Tipo del documento:
Article