Electronic cigarette vapor increases Streptococcus mutans growth, adhesion, biofilm formation, and expression of the biofilm-associated genes.
Oral Dis
; 27(3): 639-647, 2021 Apr.
Article
en En
| MEDLINE
| ID: mdl-32683796
ABSTRACT
OBJECTIVE:
It still not known whether electronic cigarettes (e-cigarettes) contribute to dental caries. This study aimed to evaluate the effect of e-cigarettes on the growth of Streptococcus mutans, the formation of biofilm, and the expression of certain virulence genes. MATERIALS ANDMETHODS:
Streptococcus mutans cells were exposed or not to e-cigarettes with and without nicotine or to cigarette smoke twice a day for 15 min each exposure period. The bacterial growth and the expression of glucosyltranferase, competence, and glucan-binding genes were evaluated after 24 hr. Biofilm formation was assessed after 1, 2, and 3 days. S. mutans adhesion and growth to e-cigarette exposed human teeth were assessed.RESULTS:
We observed an increase in S. mutans growth with e-cigarettes, mainly at the early culture period. This was confirmed by an increase of biofilm mass ranging from 8 ± 0.5 mg with the control to 47 ± 5 mg after six exposures to nicotine-rich e-cigarettes. S. mutans cells adhered better to e-cigarette exposed teeth. E-cigarettes increased the expression of glucosyltranferase, competence, and glucan-binding genes.CONCLUSIONS:
E-cigarettes increased the growth of S. mutans and the expression of virulent genes. E-cigarettes promoted the adhesion to, and formation of biofilms on teeth surfaces.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Caries Dental
/
Sistemas Electrónicos de Liberación de Nicotina
/
Cigarrillo Electrónico a Vapor
Tipo de estudio:
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Oral Dis
Asunto de la revista:
ODONTOLOGIA
Año:
2021
Tipo del documento:
Article
País de afiliación:
Canadá