Chemical modification of enzymes with activated magnetic modifier.
Biochem Biophys Res Commun
; 145(2): 908-14, 1987 Jun 15.
Article
en En
| MEDLINE
| ID: mdl-3593378
ABSTRACT
An activated magnetic modifier, which could render biological materials magnetic property, was synthesized in following two steps:
oxidation of ferrous ions (Fe2+) with hydrogen peroxide in the presence of alpha, omega-dicarboxymethylpoly(oxyethylene) (DCPEG) to obtain DCPEG-magnetite (Fe3O4); free carboxyl groups in the DCPEG-magnetite were activated with N-hydroxysuccinimide. By coupling the activated magnetic modifier to amino groups of lipase or L-asparaginase, magnetic enzymes were prepared. They dispersed stably not only in aqueous solution but also in organic solvents with high enzymic activities. Magnetic enzymes were readily recovered from reaction mixture in a magnetic field of 6000 Oe without loss of enzymic activity.
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Banco de datos:
MEDLINE
Asunto principal:
Asparaginasa
/
Lipasa
/
Magnetismo
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
1987
Tipo del documento:
Article