Mesoporous molecularly imprinted nanoparticles with peptide mimics for the detection of phenolic compounds.
Anal Chim Acta
; 1250: 340970, 2023 Apr 15.
Article
en En
| MEDLINE
| ID: mdl-36898811
ABSTRACT
Immobilized enzymes outperform free enzymes in many properties and are widely used in environmental monitoring, engineering applications, food and medical fields. Based on the developed immobilization techniques, the search for immobilization with wider applicability, lower cost and more stable enzyme properties is of significant importance. In this study, we reported a molecular imprinting strategy for immobilizing peptide mimics of DhHP-6 on mesoporous materials. The DhHP-6 molecularly imprinted polymer (MIP) showed much higher adsorption capacity than raw mesoporous silica toward DhHP-6. The DhHP-6 peptide mimics was immobilized on the surface of mesoporous silica for the fast detection of phenolic compounds, a widely spread pollutant with highly toxic and difficult in degradation. Immobilized enzyme of DhHP-6-MIP exhibited higher peroxidase activity, better stability, and recyclability than free peptide. Notably, DhHP-6-MIP showed excellent linearity for the detection of the two phenols with detection limits of 0.28 µM and 0.25 µM, respectively. In combination with the spectral analysis and PCA method, DhHP-6-MIP provided better discrimination between the six phenolic compounds (phenol, catechol, resorcinol, hydroquinone, 2-chlorophenol, 2, 4-dichlorophenol). Our study showed that immobilization of peptide mimics by the molecular imprinting strategy using mesoporous silica as carriers was a simple and effective approach. The DhHP-6-MIP has great potentiality for the monitoring and degradation of environmental pollutants.
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Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Nanopartículas
/
Impresión Molecular
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Anal Chim Acta
Año:
2023
Tipo del documento:
Article
País de afiliación:
China