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Quantitation and identification of microplastics accumulation in human placental specimens using pyrolysis gas chromatography mass spectrometry.
Garcia, Marcus A; Liu, Rui; Nihart, Alex; El Hayek, Eliane; Castillo, Eliseo; Barrozo, Enrico R; Suter, Melissa A; Bleske, Barry; Scott, Justin; Forsythe, Kyle; Gonzalez-Estrella, Jorge; Aagaard, Kjersti M; Campen, Matthew J.
Afiliación
  • Garcia MA; Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
  • Liu R; Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
  • Nihart A; Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
  • El Hayek E; Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
  • Castillo E; Division of Gastroenterology and Hepatology, Department of Internal Medicine, University of New Mexico School of Medicine, Albuquerque, New Mexico 87106, USA.
  • Barrozo ER; Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Hospital, Houston, Texas 77030, USA.
  • Suter MA; Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Hospital, Houston, Texas 77030, USA.
  • Bleske B; Department of Pharmacy Practice and Administrative Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
  • Scott J; School of Civil & Environmental Engineering, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
  • Forsythe K; School of Civil & Environmental Engineering, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
  • Gonzalez-Estrella J; School of Civil & Environmental Engineering, Oklahoma State University, Stillwater, Oklahoma 74078, USA.
  • Aagaard KM; Division of Maternal-Fetal Medicine, Department of Obstetrics and Gynecology, Baylor College of Medicine and Texas Children's Hospital, Houston, Texas 77030, USA.
  • Campen MJ; Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences, Albuquerque, New Mexico 87106, USA.
Toxicol Sci ; 199(1): 81-88, 2024 Apr 29.
Article en En | MEDLINE | ID: mdl-38366932
ABSTRACT
The exponential increase in global plastic usage has led to the emergence of nano- and microplastic (NMP) pollution as a pressing environmental issue due to its implications for human and other mammalian health. We have developed methodologies to extract solid materials from human tissue samples by saponification and ultracentrifugation, allowing for highly specific and quantitative analysis of plastics by pyrolysis-gas chromatography and mass spectrometry (Py-GC-MS). As a benchmark, placenta tissue samples were analyzed using fluorescence microscopy and automated particle count, which demonstrated the presence of >1-micron particles and fibers, but not nano-sized plastic particles. Analyses of the samples (n = 10) using attenuated total reflectance-Fourier transform infrared spectroscopy indicated presence of rayon, polystyrene, polyethylene, and unclassified plastic particles. By contrast, among 62 placenta samples, Py-GC-MS revealed that microplastics were present in all participants' placentae, with concentrations ranging widely from 6.5 to 685 µg NMPs per gram of placental tissue, averaging 126.8 ± 147.5 µg/g (mean±SD). Polyethylene was the most prevalent polymer, accounting for 54% of total NMPs and consistently found in nearly all samples (mean 68.8 ± 93.2 µg/g placenta). Polyvinyl chloride and nylon each represented approximately 10% of the NMPs by weight, with the remaining 26% of the composition represented by 9 other polymers. Together, these data demonstrate advancements in the unbiased quantitative resolution of Py-GC-MS applied to the identification and quantification of NMP species at the maternal-fetal interface. This method, paired with clinical metadata, will be pivotal to evaluating potential impacts of NMPs on adverse pregnancy outcomes.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Placenta / Microplásticos / Cromatografía de Gases y Espectrometría de Masas Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Toxicol Sci Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Placenta / Microplásticos / Cromatografía de Gases y Espectrometría de Masas Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Toxicol Sci Asunto de la revista: TOXICOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos