Unbiased phosphoproteomic method identifies the initial effects of a methacrylic acid copolymer on macrophages.
Proc Natl Acad Sci U S A
; 112(34): 10673-8, 2015 Aug 25.
Article
in En
| MEDLINE
| ID: mdl-26261332
ABSTRACT
An unbiased phosphoproteomic method was used to identify biomaterial-associated changes in the phosphorylation patterns of macrophage-like cells. The phosphorylation differences between differentiated THP1 (dTHP1) cells treated for 10, 20, or 30 min with a vascular regenerative methacrylic acid (MAA) copolymer or a control methyl methacrylate (MM) copolymer were determined by MS. There were 1,470 peptides (corresponding to 729 proteins) that were differentially phosphorylated in dTHP1 cells treated with the two materials with a greater cellular response to MAA treatment. In addition to identifying pathways (such as integrin signaling and cytoskeletal arrangement) that are well known to change with cell-material interaction, previously unidentified pathways, such as apoptosis and mRNA splicing, were also discovered.
Key words
Full text:
1
Database:
MEDLINE
Main subject:
Phosphoproteins
/
Polymethacrylic Acids
/
Biocompatible Materials
/
Protein Processing, Post-Translational
/
Gene Expression Profiling
/
Proteomics
/
Macrophages
Type of study:
Prognostic_studies
Limits:
Humans
Language:
En
Journal:
Proc Natl Acad Sci U S A
Year:
2015
Type:
Article