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Unbiased phosphoproteomic method identifies the initial effects of a methacrylic acid copolymer on macrophages.
Chamberlain, Michael Dean; Wells, Laura A; Lisovsky, Alexandra; Guo, Hongbo; Isserlin, Ruth; Talior-Volodarsky, Ilana; Mahou, Redouan; Emili, Andrew; Sefton, Michael V.
Affiliation
  • Chamberlain MD; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Wells LA; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Lisovsky A; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Guo H; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Isserlin R; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Talior-Volodarsky I; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Mahou R; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Emili A; Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada M5S 3G9;
  • Sefton MV; Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada M5S 3G9; Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON, Canada M5S 3G9 michael.sefton@utoronto.ca.
Proc Natl Acad Sci U S A ; 112(34): 10673-8, 2015 Aug 25.
Article in En | MEDLINE | ID: mdl-26261332
ABSTRACT
An unbiased phosphoproteomic method was used to identify biomaterial-associated changes in the phosphorylation patterns of macrophage-like cells. The phosphorylation differences between differentiated THP1 (dTHP1) cells treated for 10, 20, or 30 min with a vascular regenerative methacrylic acid (MAA) copolymer or a control methyl methacrylate (MM) copolymer were determined by MS. There were 1,470 peptides (corresponding to 729 proteins) that were differentially phosphorylated in dTHP1 cells treated with the two materials with a greater cellular response to MAA treatment. In addition to identifying pathways (such as integrin signaling and cytoskeletal arrangement) that are well known to change with cell-material interaction, previously unidentified pathways, such as apoptosis and mRNA splicing, were also discovered.
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Full text: 1 Database: MEDLINE Main subject: Phosphoproteins / Polymethacrylic Acids / Biocompatible Materials / Protein Processing, Post-Translational / Gene Expression Profiling / Proteomics / Macrophages Type of study: Prognostic_studies Limits: Humans Language: En Journal: Proc Natl Acad Sci U S A Year: 2015 Type: Article
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Full text: 1 Database: MEDLINE Main subject: Phosphoproteins / Polymethacrylic Acids / Biocompatible Materials / Protein Processing, Post-Translational / Gene Expression Profiling / Proteomics / Macrophages Type of study: Prognostic_studies Limits: Humans Language: En Journal: Proc Natl Acad Sci U S A Year: 2015 Type: Article