Abstract
Objective:
To evaluate the periapical healing following
root canal treatment in
teeth with
apical periodontitis (in vivo) and the cytotoxic potential of
root canal sealers
in vitro. Material and
Methods:
Apical periodontitis was induced in 60
dogs'
teeth and
root canals were filled with Sealapex (40 roots), EndoREZ (40 roots), intracanal
dressing (20 roots), or left untreated (20 roots). After 30 and 90 days, histopathological analyses were made.
In vitro, J774.1
macrophages were stimulated with
root canal sealers extracts, cytotoxicity was assessed using
lactate dehydrogenase assay, and qRT-
PCR was used to analyze TNF-α
gene expression.
Results:
In vivo, smaller
apical periodontitis and lower inflammatory
cell infiltrate were found in
teeth treated with Sealapex compared to EndoREZ.
In vitro, EndoREZ was cytotoxic and induced TNF-α
gene expression by
macrophages differently from Sealapex.
Conclusion:
Sealapex allowed improved
tissue repair following
root canal treatment in
teeth with
apical periodontitis compared to EndoREZ. Synthesis of TNF-α induced by LPS was enhanced by EndoREZ, whereas Sealapex prevented pro-inflammatory
gene expression (AU).