In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria
GRAZIANO, Talita Signoreti; CALIL, Caroline Morini; SARTORATTO, Adilson; FRANCO, Gilson César Nobre; GROPPO, Francisco Carlos; COGO-MÜLLER, Karina.
J. appl. oral sci
; 24(6): 582-589, Nov.-Dec. 2016. tab, graf
Artigo
em Inglês
| LILACS, BBO - odontologia (Brasil) | ID: biblio-841150
ABSTRACT Objective Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. Material and Methods The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. Results The MIC and MBC values were as follows M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H2S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H2S and methyl mercaptan (CH3SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H2S and CH3SH decreased (p<0.05, ANOVA-Dunnet). Conclusion M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine.
Assuntos
Compostos de Enxofre/metabolismo Porphyromonas gingivalis/efeitos dos fármacos Óleo de Melaleuca/farmacologia Melaleuca/química Porphyromonas endodontalis/efeitos dos fármacos Antibacterianos/farmacologia Compostos de Enxofre/análise Fatores de Tempo Testes de Sensibilidade Microbiana Células Cultivadas Reprodutibilidade dos Testes Análise de Variância Porphyromonas gingivalis/crescimento & desenvolvimento Porphyromonas gingivalis/metabolismo Porphyromonas endodontalis/crescimento & desenvolvimento Porphyromonas endodontalis/metabolismo Carga Bacteriana/efeitos dos fármacos Halitose/metabolismo Halitose/microbiologia Halitose/prevenção & controle Cromatografia Gasosa-Espectrometria de Massas
Biblioteca responsável:
BR1.1
powered by iAHx-2.18-86
BVS Odontologia