In Vitro antimicrobial photoinactivation with methylene blue in different microorganisms
Oliveira, Bruna Paloma de; Lins, Carla Cabral dos Santos Accioly; Diniz, Fátima Alves; Melo, Liliane Lima; Castro, Célia Maria Machado Barbosa de.
Braz. j. oral sci
; 13(1): 53-57, Jan-Mar/2014. tab, graf
Artigo
em Inglês
| LILACS | ID: lil-709499
AIM:
To evaluate the in vitro antimicrobial effects of photodynamic therapy (PDT).METHODS:
The microorganism indicators were Candida albicans, Pseudomonas aeruginosa,Enterococcus faecalis and Staphylococcus aureus. A microbial pool was prepared (10 8 cells/mL), from which aliquots were transferred to culture plates for carrying out the PDT using methylene blue (50 µM) and low-power laser (660 nm, 100 mW and 9 J).The effect of methylene blue alone, low power laser and the absence of treatments were evaluated. Then, aliquots of 1 µL were plated in a media culture, the number of colony forming units (CFU/mL) was obtained and the data submitted to the F test (ANOVA) with Tamhane's comparisons.RESULTS:
The laser radiation in the presence of methylene blue was able to eliminate 74.90% of C. albicans, 72.41% of P. aeruginosa, 96.44% of E. faecalis and 95.42% of S. aureus, thus statistically significant differences were found among the groups (p<0.001).CONCLUSIONS:
PDT was effective in reducing the number of viable cells in the studiedmicroorganisms, especially E. faecalis and S. aureus.
Biblioteca responsável:
BR218.1
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