Inhibition of eukaryotic dna polymerase alpha by persimmon (Diospyros kaki) extract and related polyphenols.
Biochem Mol Biol Int
; 47(5): 795-801, 1999 May.
Article
em En
| MEDLINE
| ID: mdl-10365250
ABSTRACT
The effects of persimmon extract (Diospyros kaki) and related polyphenols on eukaryotic DNA polymerase alpha were examined. It was found that persimmon extract, epigallocatechin gallate, and epicatechin gallate strongly inhibited the activity of DNA polymerase alpha purified from calf thymus. Among these polyphenols, persimmon extract had the most potent effect on DNA polymerase alpha activity and the concentration of persimmon extract producing 50% inhibition of the activity was 0.191 microM. Persimmon extract showed a weaker effect on DNA polymerase beta and slightly inhibited primase and DNA polymerase I. The inhibition of DNA polymerase alpha by persimmon extract was competitive with the template-primer and noncompetitive with dTTP substrate. The Ki value of DNA polymerase alpha for persimmon extract was estimated to be 70 nM. Moreover, persimmon extract inhibited [3H]thymidine incorporation of human peripheral lymphocyte cells stimulated by PHA.
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Base de dados:
MEDLINE
Assunto principal:
Fenóis
/
Proteínas de Plantas
/
Polímeros
/
Flavonoides
/
DNA Polimerase I
Limite:
Humans
Idioma:
En
Revista:
Biochem Mol Biol Int
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
1999
Tipo de documento:
Article
País de afiliação:
Japão