The effect of lexA and recF mutations on post-replication repair and DNA synthesis in Escherichia coli K-12.
Mol Gen Genet
; 141(3): 189-205, 1975 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-1107815
ABSTRACT
We have examined lexA1 uvrA6 and recF143 uvrBdelta derivatives of Escherichia coli K-12 for post-replication repair and DNA synthesis after UV irradiation. Compared to corresponding lex+ rec+ strains, we found that the lexA and recF cells were defective in (1) converting short DNA segments synthesized after irradiation to DNA of normal size; (2) synthesizing high molecular weight DNA after irradiation; (3) transferring pyrimidine dimers from irradiated DNA into unirradiated daughter strands. Our results support the hypothesis that after UV irradiation the formation of large DNA molecules in excision-deficient cells of E.coli depends directly or indirectly upon joining short DNA segments into longer strands, concomitant with the transfer of DNA from irradiated tamplates into unirradiated daughter strands. This process appears to require the activity of lexA and recF genes.
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Base de dados:
MEDLINE
Assunto principal:
DNA Bacteriano
/
Reparo do DNA
/
Escherichia coli
Idioma:
En
Revista:
Mol Gen Genet
Ano de publicação:
1975
Tipo de documento:
Article