Purification of a novel ribonuclease from dried fruiting bodies of the edible wild mushroom Thelephora ganbajun.
Biochem Biophys Res Commun
; 324(2): 855-9, 2004 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-15474506
ABSTRACT
A ribonuclease, with a molecular mass of 30 kDa and a potent inhibitory activity toward HIV-1 reverse transcriptase (IC50=300 nM), was isolated from dried fruiting bodies of the edible wild mushroom Thelephora ganbajun. The ribonuclease exhibited a unique polyhomoribonucleotide specificity, with the highest activity toward poly(U), about 50% and 25% as much activity toward poly(A) and poly(C), respectively, and minimal activity toward poly(G). Unlike other mushroom RNases, the ribonuclease was adsorbed on DEAE-cellulose and Q-Sepharose, and unadsorbed on CM-cellulose. A temperature of 40 degrees C and a pH of 6-7 were required for maximal activity of the enzyme. The enzyme was characterized by an N-terminal sequence without any homology to known proteins.
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Base de dados:
MEDLINE
Assunto principal:
Ribonucleases
/
Basidiomycota
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China