Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression.
Int J Oral Maxillofac Implants
; 21(2): 203-11, 2006.
Article
em En
| MEDLINE
| ID: mdl-16634490
ABSTRACT
PURPOSE:
The objective of this study was to test the hypothesis that fluoride-modified titanium surfaces would enhance osteoblast differentiation. Osteoblast growth on a moderately rough etched fluoride-modified titanium surface (alteration in cellular differentiation) was compared to osteoblast growth on the same surface grit-blasted with titanium dioxide. The potential role of nanometer-level alterations on cell shape and subsequent differentiation was then compared. MATERIALS ANDMETHODS:
Human embryonic palatal mesenchymal (HEPM) cultures were incubated on the respective surfaces for 1, 3, and 7 days, followed by analysis for cell proliferation, alkaline phosphatase (ALP) -specific activity, and mRNA steady-state expression for bone-related genes (ALP, type I collagen, osteocalcin, bone sialoprotein [BSP] II, Cbfa1, and osterix) by real-time polymerase chain reaction (PCR).RESULTS:
The different surfaces did not alter the mRNA expression for ALP, type I collagen, osterix, osteocalcin, or BSP II. However, Cbfa1 expression on the fluoride-modified titanium surface was significantly higher (P < .001) at 1 week. The number of cells on this surface was 20% lower than the number of cells on the surface TiO2-blasted with 25-microm particles but not significantly different from the number of cells on the surface TiO2-blasted with 125-microm particles. Cells grown on all the titanium surfaces expressed similar levels of ALP activity.CONCLUSIONS:
The results indicated that a fluoride-modified surface topography, in synergy with surface roughness, may have a greater influence on the level of expression of Cbfa1 (a key regulator for osteogenesis) than the unmodified titanium surfaces studied.
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Titânio
/
Adesão Celular
/
Subunidade alfa 1 de Fator de Ligação ao Core
/
Fluoretos
Limite:
Humans
Idioma:
En
Revista:
Int J Oral Maxillofac Implants
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Malásia