Multicolor fluorescence in situ hybridization and pulsed field electrophoresis dissect CMT1B gene region.
Hum Genet
; 88(1): 13-20, 1991 Nov.
Article
em En
| MEDLINE
| ID: mdl-1683644
ABSTRACT
We have used multicolor fluorescence in situ hybridization of banded chromosomes to orient Fc gamma RII and clone 1054 on a single early metaphase chromosome band (1q22) representing about 2% of the physical map of chromosome 1 in the Charcot-Marie-Tooth (CMT1B) gene region. These two cloned fragments are on the same partially digested 900-kb MluI fragment detected by pulsed field gel electrophoresis. When applied to data from an earlier study, multicolor in situ hybridization results further refined the CMT1B genetic location from an 18 cM interval to a 6 cM interval and the physical map from 15% of chromosome 1 to 3% of chromosome 1. Occasionally the three Fc gamma RII immunoglobulin receptor genes within the 200-kb region are resolved in individual metaphase chromatids.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 1
/
Doença de Charcot-Marie-Tooth
Limite:
Humans
Idioma:
En
Revista:
Hum Genet
Ano de publicação:
1991
Tipo de documento:
Article