Development and validation of a rapid reversed-phase HPLC method for the determination of the non-nucleoside reverse transcriptase inhibitor dapivirine from polymeric nanoparticles.
J Pharm Biomed Anal
; 52(2): 167-72, 2010 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-20117900
ABSTRACT
The objective of this work was to develop and validate a rapid reversed-phase (RP) high-performance liquid chromatography (HPLC) method for the in vitro pharmaceutical characterization of dapivirine-loaded polymeric nanoparticles. Chromatographic runs were performed on a RP C18 column with a mobile phase comprising acetonitrile-0.5% (w/v) triethanolamine solution in isocratic mode (8020, v/v) at a flow rate of 1 ml/min. Dapivirine was detected at a wavelength of 290 nm. The method was shown to be specific, linear in the range of 1-50 microg/ml (R(2)=0.9998), precise at the intra-day and inter-day levels as reflected by the relative standard deviation values (less than 0.85%), accurate (recovery rate of 100.17+/-0.35%), and robust to changes in the mobile phase and column brand. The detection and quantitation limits were 0.08 and 0.24 microg/ml, respectively. The method was successfully used to determine the loading capacity and association efficiency of dapivirine in poly(lactic-co-glycolic acid)-based nanoparticles and its in vitro release.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polímeros
/
Pirimidinas
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Cromatografia Líquida de Alta Pressão
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Nanopartículas
/
Transcriptase Reversa do HIV
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2010
Tipo de documento:
Article
País de afiliação:
Portugal