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Elimination of isobaric interference and signal-to-noise ratio enhancement using on-line mobile phase filtration in liquid chromatography/tandem mass spectrometry.

Lahaie, Mathieu; Youhnovski, Nikolay; Furtado, Milton; Garofolo, Fabio.
Rapid Commun Mass Spectrom; 28(8): 886-92, 2014 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-24623692
RATIONALE Liquid chromatography/tandem mass spectrometry (LC/MS/MS) instruments are selective and sensitive but can still be affected by isobaric interference or chemical noise arising from multiple sources such as the mobile phase. In this study, a high-performance liquid chromatography (HPLC) on-line mobile phase filtration setup is described and used to remove interference to allow better detection of the analyte of interest.


For instance, a filtration device containing a chemical sorbent is installed at the HPLC outlet of the aqueous solvent pump A or the organic solvent pump B. This manuscript reports different case scenarios under reversed-phase and HILIC separations either in positive (ESI(+)) or negative electrospray ionization (ESI(-)) mode using selected reaction monitoring (SRM) scans as well as additional Q1 MS scans.


The filtration of the aqueous effluent of the mobile phase using a porous graphitic carbon filter eliminated the isobaric interferences and improved the detectability of gestodene and perindopril-D4. Also, a strong cation-exchange guard column installed at the acetonitrile outlet pump was found helpful on reducing the baseline intensity and improving significantly the signal-to-noise ratio (S/N) of methenamine. Moreover, the on-line mobile phase filtration was efficient at removing chemical background ions in full scan mode.


This strategy demonstrated its usefulness by removing co-eluting isobaric interference, and reducing chemical background ions from the mobile phase, while drastically improving S/N.