Odontoblast ß-catenin signaling regulates fenestration of mouse Hertwig's epithelial root sheath.
Sci China Life Sci
; 58(9): 876-81, 2015 Sep.
Article
em En
| MEDLINE
| ID: mdl-26208822
ABSTRACT
The interaction between Hertwig's epithelial root sheath (HERS) and the adjacent mesenchyme is vitally important in mouse tooth root development. We previously generated odontoblast-specific Ctnnb1 (encoding ß-catenin) deletion mice, and demonstrated that odontoblast ß-catenin signaling regulates odontoblast proliferation and differentiation. However, the role of odontoblast ß-catenin signaling in regulation of HERS behavior has not been fully investigated. Here, using the same odontoblast- specific Ctnnb1 deletion mice, we found that ablation of ß-catenin signaling in odontoblasts led to aberrant HERS formation. Mechanistically, odontoblast-specific Ctnnb1 deletion resulted in elevated bone morphogenetic protein 7 (Bmp7) expression and reduced expression of noggin and follistatin, both of which encode extracellular inhibitors of BMPs. Furthermore, the levels of phosphorylated Smad1/5/8 were increased in HERS cells. In vitro tissue culture confirmed that BMP7 treatment disrupted the HERS structure. Taken together, we demonstrated that odontoblast ß-catenin signaling may act through regulation of BMP signaling to maintain the integrity of HERS cells.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Raiz Dentária
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Órgão do Esmalte
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Beta Catenina
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Mesoderma
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Odontoblastos
Limite:
Animals
Idioma:
En
Revista:
Sci China Life Sci
Assunto da revista:
BIOLOGIA
/
CIENCIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China