In vitro studies on human periodontal ligament stem cell sheets enhanced by enamel matrix derivative.
Colloids Surf B Biointerfaces
; 141: 102-111, 2016 May 01.
Article
em En
| MEDLINE
| ID: mdl-26844646
ABSTRACT
Numerous preclinical and clinical studies have focused on the periodontal regenerative functions of enamel matrix derivative (EMD), a heat-treated preparation derived from enamel matrix proteins (EMPs) of developing porcine teeth. In this study, periodontal ligament (PDL) stem cells (PDLSCs) were isolated, and the effects of EMD on the extracorporeal induction process and the characteristics of PDLSC sheets were investigated for their potential as a more effective stem-cell therapy. EMD-enhanced cell sheets could be induced by complete medium supplemented with 50 µg/mL vitamin C and 100 µg/mL EMD. The EMD-enhanced cell sheets appeared thicker and more compact than the normal PDLSC sheets, demonstrated more layers of cells (3-7 layers), secreted richer extracellular matrix (ECM), showed varying degrees of increases in mRNA expression of periodontal tissue-specific genes (COL I, POSTN), calcification-related genes (RUNX2, OPN, OCN) and a cementum tissue-specific gene (CAP), and possessed a better mineralization ability in terms of osteogenic differentiation in vitro. These EMD-enhanced cell sheets may represent a potential option for stem-cell therapy for PDL regeneration.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ligamento Periodontal
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Células-Tronco
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Proteínas do Esmalte Dentário
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Proliferação de Células
Limite:
Adult
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Animals
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Humans
Idioma:
En
Revista:
Colloids Surf B Biointerfaces
Assunto da revista:
QUIMICA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
China