Lipids Cooperate with the Reovirus Membrane Penetration Peptide to Facilitate Particle Uncoating.
J Biol Chem
; 291(52): 26773-26785, 2016 Dec 23.
Article
em En
| MEDLINE
| ID: mdl-27875299
ABSTRACT
Virus-host interactions play a role in many stages of the viral lifecycle, including entry. Reovirus, a model system for studying the entry mechanisms of nonenveloped viruses, undergoes a series of regulated structural transitions that culminate in delivery of the viral genetic material. Lipids can trigger one of these conformational changes, infectious subviral particle (ISVP)-to-ISVP* conversion. ISVP* formation releases two virally encoded peptides, myristoylated µ1N (myr-µ1N) and Φ. Among these, myr-µ1N is sufficient to form pores within membranes. Released myr-µ1N can also promote ISVP* formation in trans Using thermal inactivation as a readout for ISVP-to-ISVP* conversion, we demonstrate that lipids render ISVPs less thermostable in a virus concentration-dependent manner. Under conditions in which neither lipids alone nor myr-µ1N alone promotes ISVP-to-ISVP* conversion, myr-µ1N induces particle uncoating when lipids are present. These data suggest that the pore-forming activity and the ISVP*-promoting activity of myr-µ1N are linked. Lipid-associated myr-µ1N interacts with ISVPs and triggers efficient ISVP* formation. The cooperativity between a reovirus component and lipids reveals a distinct virus-host interaction in which membranes can facilitate nonenveloped virus entry.
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MEDLINE
Assunto principal:
Reoviridae
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Vírion
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Membrana Celular
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Infecções por Reoviridae
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Proteínas do Capsídeo
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Peptídeos Penetradores de Células
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Lipídeos de Membrana
Limite:
Animals
Idioma:
En
Revista:
J Biol Chem
Ano de publicação:
2016
Tipo de documento:
Article