Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months.
Nat Commun
; 7: 13520, 2016 11 30.
Article
em En
| MEDLINE
| ID: mdl-27901019
ABSTRACT
Up to 80% of the cost of vaccination programmes is due to the cold chain problem (that is, keeping vaccines cold). Inexpensive, biocompatible additives to slow down the degradation of virus particles would address the problem. Here we propose and characterize additives that, already at very low concentrations, improve the storage time of adenovirus type 5. Anionic gold nanoparticles (10-8-10-6 M) or polyethylene glycol (PEG, molecular weight â¼8,000 Da, 10-7-10-4 M) increase the half-life of a green fluorescent protein expressing adenovirus from â¼48 h to 21 days at 37 °C (from 7 to >30 days at room temperature). They replicate the known stabilizing effect of sucrose, but at several orders of magnitude lower concentrations. PEG and sucrose maintained immunogenicity in vivo for viruses stored for 10 days at 37 °C. To achieve rational design of viral-vaccine stabilizers, our approach is aided by simplified quantitative models based on a single rate-limiting step.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Estabilidade de Medicamentos
/
Excipientes
/
Vacinas contra Adenovirus
/
Imunogenicidade da Vacina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Nat Commun
Assunto da revista:
BIOLOGIA
/
CIENCIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Itália