Assessing Collagen and Micro-permeability at the Proanthocyanidin-treated Resin-Dentin Interface.
J Adhes Dent
; 18(6): 529-534, 2016.
Article
em En
| MEDLINE
| ID: mdl-27933323
ABSTRACT
PURPOSE:
To establish a fluorescence-based method to simultaneously assess micro-permeability and collagen cross-linking induced by chemical agents at the resin-dentin interface. MATERIALS ANDMETHODS:
Three chemical agents were investigated (proanthocyanidin-rich grape seed extract GSE; carbodiimide hydrochloride/N-hydroxysuccinimide EDC/NHS; glutaraldehyde GD) along with a control (distilled water) as primers applied on flat occlusal dentin surfaces of 48 teeth and restored with two commercially available etch-and-rinse adhesives. Resin-dentin interfaces were polished and infiltrated with rhodamine-B solution for confocal laser scanning microscopy analysis. Parameters were chosen that would allow acquisition of a simultaneous appearance of collagen and interfacial micro-permeability (rhodamine-B). Fluorescence emission intensity (FEI) was converted into numerals and values were calculated for each group. Data were statistically analyzed using one-way ANOVA and post-hoc Scheffe's and multiple comparisons tests (α = 0.05). T-tests with Pearson correlations were used to investigate correlations between collagen cross-linking and micro-permeability.RESULTS:
The FEI of collagen was the highest for GD, followed by GSE, with no significant differences between EDC/ NHS and the control group (p > 0.05). Micro-permeability was significantly affected by the adhesives (p < 0.05). Micro- permeability was the lowest for GSE groups, regardless of the adhesives (p < 0.001). Weak correlations were found between micro-permeability and collagen auto-fluorescence.CONCLUSIONS:
Non-enzymatic collagen cross-linking induced by GSE and GD can be detected by increased collagen auto-fluorescence, and results in reduced interfacial micro-permeability. Increased collagen auto-fluorescence was correlated with fluorescent collagen cross-links and decreased micro-permeability at the resin-dentin interface. Collagen auto-fluorescence is a useful tool to detect auto-fluorescent exogenous cross links and their potential impact on the quality of the resin-dentin interface.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Resinas Sintéticas
/
Colágeno
/
Proantocianidinas
/
Dentina
Limite:
Humans
Idioma:
En
Revista:
J Adhes Dent
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article