Hydrothermal derived protoporphyrin IX nanoparticles for inactivation and imaging of bacteria strains.
J Colloid Interface Sci
; 549: 72-79, 2019 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-31022525
ABSTRACT
Overuse and abuse of antibiotics greatly hasten the development of microbial drug resistance and substantially threat to global public health. Developing alternative methods for combating bacterial infections is urgently required. In this work, a simple hydrothermal approach was employed to prepare the protoporphyrin IX-polyethylenimine nanoparticles (PPIX-PEI NPs) containing abundant amine groups and PPIX moieties. The as-obtained PPIX-PEI NPs exhibit antibacterial properties against both Gram-positive and Gram-negative bacteria. The presence of PPIX in the PPIX-PEI NPs can generate reactive oxygen species (ROS) under 635â¯nm laser irradiation, which enhance the antibacterial properties of the PPIX-PEI NPs against Gram-positive bacteria. Thus, the PPIX-PEI NPs display a synergistic antibacterial activity against Gram-positive bacteria in the combination of antibacterial photodynamic therapy (PDT). In addition, emission of red fluorescence by the PPIX-PEI NPs can help to differentiate bacteria and observe the bacterial morphologies using a confocal laser scanning microscope (CLSM).
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Base de dados:
MEDLINE
Assunto principal:
Protoporfirinas
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Fármacos Fotossensibilizantes
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Nanopartículas
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Antibacterianos
Limite:
Humans
Idioma:
En
Revista:
J Colloid Interface Sci
Ano de publicação:
2019
Tipo de documento:
Article