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IL-37 inhibits M1-like macrophage activation to ameliorate temporomandibular joint inflammation through the NLRP3 pathway.
Luo, Ping; Peng, Sisi; Yan, Yin; Ji, Ping; Xu, Jie.
Afiliação
  • Luo P; Department of Oral and Maxillofacial Surgery, College of Stomatology, Chongqing Medical University.
  • Peng S; Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences.
  • Yan Y; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
  • Ji P; Department of Oral and Maxillofacial Surgery, College of Stomatology, Chongqing Medical University.
  • Xu J; Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences.
Rheumatology (Oxford) ; 59(10): 3070-3080, 2020 10 01.
Article em En | MEDLINE | ID: mdl-32417913
ABSTRACT

OBJECTIVES:

IL-37 has been identified as an important anti-inflammatory and immunosuppressive factor. This study was undertaken to explore how IL-37 affects M1/M2-like macrophage polarization and thus contributes to anti-inflammatory processes in the temporomandibular joint.

METHODS:

Western blotting, quantitative real-time PCR (qRT-PCR) and immunofluorescence were used to verify the IL-37-induced polarization shift from the M1 phenotype to the M2 phenotype, and the related key pathways were analysed by western blotting. Human chondrocytes were stimulated with M1-conditioned medium (CM) or IL-37-pretreated M1-CM, and inflammatory cytokines were detected. siRNA-IL-1R8 and MCC-950 were used to investigate the mechanism underlying the anti-inflammatory effects of IL-37. Complete Freund's adjuvant-induced and disc perforation-induced inflammation models were used for in vivo studies. Haematoxylin and eosin, immunohistochemical and safranin-O staining protocols were used to analyse histological changes in the synovium and condyle.

RESULTS:

Western blotting, qRT-PCR and immunofluorescence showed that IL-37 inhibited M1 marker expression and upregulated M2 marker expression. Western blotting and qRT-PCR showed that pretreatment with IL-37 suppressed inflammatory cytokine expression in chondrocytes. IL-37 inhibited the expression of NLRP3 and upregulated the expression of IL-1R8. Si-IL-1R8 and MCC-950 further confirmed that the anti-inflammatory properties of IL-37 were dependent on the presence of IL-1R8 and NLRP3. In vivo, IL-37 reduced synovial M1 marker expression and cartilage degeneration and increased M2 marker expression.

CONCLUSION:

IL-37 shifting of the polarization of macrophages from the pro-inflammatory M1 phenotype to the beneficial anti-inflammatory M2 phenotype seems to be a promising therapeutic strategy for treating temporomandibular joint inflammation.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transtornos da Articulação Temporomandibular / Interleucina-1 / Polaridade Celular / Proteína 3 que Contém Domínio de Pirina da Família NLR / Ativação de Macrófagos / Macrófagos Limite: Humans Idioma: En Revista: Rheumatology (Oxford) Assunto da revista: REUMATOLOGIA Ano de publicação: 2020 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transtornos da Articulação Temporomandibular / Interleucina-1 / Polaridade Celular / Proteína 3 que Contém Domínio de Pirina da Família NLR / Ativação de Macrófagos / Macrófagos Limite: Humans Idioma: En Revista: Rheumatology (Oxford) Assunto da revista: REUMATOLOGIA Ano de publicação: 2020 Tipo de documento: Article