Your browser doesn't support javascript.
loading
Different grinding speeds affect induced regeneration capacity of human treated dentin matrix.
Li, Min; Yang, Sen; Song, Jinlin; Fu, Tiwei; Liang, Panpan; Gao, Zhi; Tang, Jing; Guo, Lijuan.
Afiliação
  • Li M; Department of Stomatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
  • Yang S; Department of Medical Cosmetology, Suining Central Hospital, Suining, China.
  • Song J; Department of Stomatology, Xinqiao Hospital, Army Medical University (Third Military Medical University), Chongqing, China.
  • Fu T; Stomatology Center, Suining Central Hospital, Suining, China.
  • Liang P; Chongqing Medical University Stomatology College, Chongqing, China.
  • Gao Z; Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing, China.
  • Tang J; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing, China.
  • Guo L; Chongqing Medical University Stomatology College, Chongqing, China.
J Biomed Mater Res B Appl Biomater ; 110(4): 755-767, 2022 04.
Article em En | MEDLINE | ID: mdl-34637601
ABSTRACT
Human-treated dentin matrix (hTDM) is a biomaterial scaffold, which can induce implant cells to differentiate into odontoblasts and then form neo-dentin. However, hTDM with long storage or prepared by high-speed handpiece would not to form neo-dentin. In this research, we developed two fresh hTDM with different grinding speeds, which were low-speed hTDM (LTDM) with maximum speed of 500 rpm and high-speed hTDM (HTDM) with a speed of 3,80,000 rpm. Here, we aim to understand whether there were induced regeneration capacity differences between LTDM and HTDM. Scanning electron microscope showed that DFCs grew well on both materials, but the morphology of DFCs and the extracellular matrix was different. Especially, the secreted extracellular matrixes on the inner surface of LTDM were regular morphology and ordered arrangement around the dentin tubules. The transcription-quantitative polymerase chain reaction (qRT-PCR), western blot and immunofluorescence assay showed that the dentin markers DSPP and DMP-1 were about 2× greater in DFCs induced by LTDM than by HTDM, and osteogenic marker BSP was about 2× greater in DFCs induced by HTDM than by LTDM. Histological examinations of the harvested grafts observed the formation of neo-tissue were different, and there were neo-dentin formed on the inner surface of LTDM and neo-cementum formed on the outer surface of HTDM. In summary, it found that the induction abilities of LTDM and HTDM are different, and the dentin matrix is directional. This study lays a necessary foundation for searching the key factors of dentin regeneration in future.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dentina / Matriz Extracelular Limite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Dentina / Matriz Extracelular Limite: Humans Idioma: En Revista: J Biomed Mater Res B Appl Biomater Assunto da revista: ENGENHARIA BIOMEDICA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China