Your browser doesn't support javascript.
loading
Mechanistic study of ultrasound-assisted chromatography using plastic and stainless steel columns.
Van Kien, Nguyen; Jeong, Young Han; Seog, David Jin Han; Ryoo, Jae Jeong.
Afiliação
  • Van Kien N; Department of Chemistry Education, Kyungpook National University, Daegu, 702-701, the Republic of Korea South Korea.
  • Jeong YH; Department of Chemistry Education, Kyungpook National University, Daegu, 702-701, the Republic of Korea South Korea.
  • Seog DJH; Department of Chemistry Education, Kyungpook National University, Daegu, 702-701, the Republic of Korea South Korea.
  • Ryoo JJ; Department of Chemistry Education, Kyungpook National University, Daegu, 702-701, the Republic of Korea South Korea. Electronic address: jjryoo@knu.ac.kr.
J Chromatogr A ; 1710: 464402, 2023 Nov 08.
Article em En | MEDLINE | ID: mdl-37797422
ABSTRACT
Based on the previous ultrasound-assisted chromatography (UAC) studies on plastic and stainless steel (SS) columns, this study explores the UAC mechanism by comparatively analyzing ultrasound effects on plastic and SS columns with C18 stationary phase when separating a mixture of polycyclic aromatic hydrocarbons (PAHs) under various ultrasound intensities. The results showed a substantial difference in H values between the PEEK and SS columns under the influence of ultrasound agitation. Specifically, for the pyrene peak, as the ultrasonic intensity increased from 0% to 100% of 900 W, the H values of the SS column slightly rose from 8.82 µm to 9.86 µm. Conversely, the corresponding values for the PEEK column exhibited a significant 12-fold increase from 11.5 µm to 134 µm. The findings demonstrated poor penetration of ultrasound energy through the SS column, and the temperature rise of the medium induced by the ultrasound was the primary contributing factor to PAH separation. However, ultrasound easily penetrated through the plastic column, resulting in acoustic cavitation within the C18 polyether ether ketone (PEEK) column. Cavitation induced heat generation and contributed to a decrease in retention time and the magnitude of peak broadening or distortion, depending on the specific ultrasonic energy. Based on the estimated change in inlet temperature of the PEEK column due to an acoustic effect, the comparison with temperature effects under non-sonic conditions consistently demonstrated a stronger acoustic effect in reducing the retention time, by 2-9%, depending on specific peaks and pairs. We revisited the previously described separation mechanism of ultrasound-assisted ion chromatography and conjoined with our findings to infer and establish a thorough explanation for the previously unexplained separation mechanism of chiral separation and size exclusion chromatography by UAC using SS columns.
Assuntos
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polímeros / Aço Inoxidável Idioma: En Revista: J Chromatogr A Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Polímeros / Aço Inoxidável Idioma: En Revista: J Chromatogr A Ano de publicação: 2023 Tipo de documento: Article