Your browser doesn't support javascript.
loading
Molecular insights into the proteomic composition of porcine treated dentin matrix.
Zhang, Xiya; Zhou, Sha; Zhan, Yuzhen; Mei, Ziyi; Qian, Aizhuo; Yuan, Yu; Zhang, Xiaonan; Fu, Tiwei; Ma, Shiyong; Li, Jie.
Afiliação
  • Zhang X; College of Stomatology, Chongqing Medical University, Chongqing, China.
  • Zhou S; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, China.
  • Zhan Y; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China.
  • Mei Z; College of Stomatology, Chongqing Medical University, Chongqing, China.
  • Qian A; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, China.
  • Yuan Y; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China.
  • Zhang X; College of Stomatology, Chongqing Medical University, Chongqing, China.
  • Fu T; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, China.
  • Ma S; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China.
  • Li J; College of Stomatology, Chongqing Medical University, Chongqing, China.
Mater Today Bio ; 25: 100990, 2024 Apr.
Article em En | MEDLINE | ID: mdl-38371466
ABSTRACT

Background:

Human-treated dentin matrix (hTDM) has recently been studied as a natural extracellular matrix-based biomaterial for dentin pulp regeneration. However, porcine-treated dentin matrix (pTDM) is a potential alternative scaffold due to limited availability. However, there is a dearth of information regarding the protein composition and underlying molecular mechanisms of pTDM.

Methods:

hTDM and pTDM were fabricated using human and porcine teeth, respectively, and their morphological characteristics were examined using scanning electron microscopy. Stem cells derived from human exfoliated deciduous teeth (SHEDs) were isolated and characterized using flow cytometry and multilineage differentiation assays. SHEDs were cultured in three-dimensional environments with hTDM, pTDM, or biphasic hydroxyapatite/tricalcium phosphate. The expression of odontogenesis markers in SHEDs were assessed using real-time polymerase chain reaction and immunochemical staining. Subsequently, SHEDs/TDM and SHEDs/HA/TCP complexes were transplanted subcutaneously into nude mice. The protein composition of pTDM was analyzed using proteomics and compared to previously published data on hTDM.

Results:

pTDM and hTDM elicited comparable upregulation of odontogenesis-related genes and proteins in SHEDs. Furthermore, both demonstrated the capacity to stimulate root-related tissue regeneration in vivo. Proteomic analysis revealed the presence of 278 protein groups in pTDM, with collagens being the most abundant. Additionally, pTDM and hTDM shared 58 identical proteins, which may contribute to their similar abilities to induce odontogenesis.

Conclusions:

Both hTDM and pTDM exhibit comparable capabilities in inducing odontogenesis, potentially owing to their distinctive bioactive molecular networks.
Palavras-chave

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Mater Today Bio Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Mater Today Bio Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China