Osteoblast differentiation of Gli1⺠cells via Wnt and BMP signaling pathways during orthodontic tooth movement.
J Oral Biosci
; 66(2): 373-380, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38499228
ABSTRACT
OBJECTIVES:
Factors that induce bone formation during orthodontic tooth movement (OTM) remain unclear. Gli1 was recently identified as a stem cell marker in the periodontal ligament (PDL). Therefore, we evaluated the mechanism of differentiation of Cre/LoxP-mediated Gli1/Tomato+ cells into osteoblasts during OTM.METHODS:
After the final administration of tamoxifen to 8-week-old Gli1-CreERT2/ROSA26-loxP-stop-loxP-tdTomato mice for 2 days, nickel-titanium closed coil springs were attached between the upper anterior alveolar bone and the first molar. Immunohistochemical localizations of ß-catenin, Smad4, and Runx2 were observed in the PDL on 2, 5, and 10 days after OTM initiation.RESULTS:
In the untreated tooth, few Gli1/Tomato+ cells were detected in the PDL. Two days after OTM initiation, the number of Gli1/Tomato+ cells increased in the PDL on the tension side. On this side, 49.3 ± 7.0% of ß-catenin+ and 48.7 ± 5.7% of Smad4+ cells were found in the PDL, and Runx2 expression was detected in some Gli1/Tomato+ cells apart from the alveolar bone. The number of positive cells in the PDL reached a maximum on day 5. In contrast, on the compression side, ß-catenin and Smad4 exhibited less immunoreactivity. On day 10, Gli1/Tomato+ cells were aligned on the alveolar bone on the tension side, with some expressing Runx2.CONCLUSIONS:
Gli1+ cells in the PDL differentiated into osteoblasts during OTM. Wnt and bone morphogenetic proteins signaling pathways may be involved in this differentiation.Palavras-chave
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
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Técnicas de Movimentação Dentária
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Diferenciação Celular
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Proteína GLI1 em Dedos de Zinco
Limite:
Animals
Idioma:
En
Revista:
J Oral Biosci
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Japão