Evaluation of anti-AIDS drugs in conventional mice implanted with a permeable membrane device containing human T cells infected with HIV.
Antiviral Res
; 35(2): 123-9, 1997 Jul.
Article
em En
| MEDLINE
| ID: mdl-9217249
ABSTRACT
We now report the confirmation of the work of Hollingshead et al. (1995) on development of a cell based hollow fiber (HF) system for evaluating potential anti-AIDS drugs in vivo using conventional mice rather than SCID mice. CD4 +, CEM-SS cells infected with HIV/1, strain RF, at a multiplicity of infection of 0.1 were placed into HFs. The fibers were implanted into the peritoneal cavity of outbred Swiss mice. Using this model, the antiviral activity of azidothymidine (AZT) at doses of approximately 150, 75 and 37.5 mg/kg/day was evaluated by administering AZT to the mice in drinking water. Upon fiber removal on day 6, AZT treatment was shown to significantly increase CEM cell viability over the untreated, virus control group and significantly reduced the levels of HIV p24 and HIV RT activity.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos T CD4-Positivos
/
HIV-1
/
Fármacos Anti-HIV
/
Avaliação Pré-Clínica de Medicamentos
/
Membranas Artificiais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Antiviral Res
Ano de publicação:
1997
Tipo de documento:
Article
País de afiliação:
Estados Unidos