RESUMEN
Oxidative DNA damage and its repair in primary rat hepatocyte cultures was investigated following 4 h of incubation with the toxic iron chelate, ferric nitrilotriacetate (Fe-NTA), in the presence or absence of the potent protective flavonoid myricetin (25-50-100 microM). Seven DNA base oxidation products were quantified in DNA extracts by gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring mode. Concomitantly, DNA repair capacity of hepatocytes was estimated by the release of oxidized-base products into culture media, using the same GC-MS method. A genotoxic effect of Fe-NTA (100 microM) in hepatocytes was evidenced by a severe increase in DNA oxidation over basal levels, with accumulation in cellular DNA of five oxidation products derived from both purines and pyrimidines. This prooxidant effect of iron was also noted by an induction of lipid peroxidation, estimated by free malondialdehyde production. Addition of increasing concentrations of myricetin (25-50-100 microM) simultaneously with iron prevented both lipid peroxidation and accumulation of oxidation products in DNA. Moreover, as an activation of DNA repair pathways, myricetin stimulated the release of DNA oxidation bases into culture media, especially of purine-derived oxidation products. This removal of highly mutagenic oxidation products from DNA of hepatocytes might correspond to an activation of DNA excision-repair enzymes by myricetin. This was verified by RNA blot analysis of DNA polymerase beta gene expression which was induced by myricetin in a dose-dependent manner. This represented a novel and original mechanism of cytoprotection by myricetin against iron-induced genotoxicity via stimulation of DNA repair processes. Since iron-induced DNA damage and inefficient repair in hepatocytes could be related to genotoxicity and most probably to hepatocarcinogenesis, modulation of these processes in vitro by myricetin might be relevant in further prevention of liver cancer derived from iron overload pathologies.
Asunto(s)
Daño del ADN , Flavonoides/farmacología , Hierro/farmacología , Hígado/efectos de los fármacos , Animales , Células Cultivadas , Reparación del ADN , Cromatografía de Gases y Espectrometría de Masas , L-Lactato Deshidrogenasa/metabolismo , Peroxidación de Lípido/efectos de los fármacos , Hígado/citología , Masculino , Malondialdehído/metabolismo , Pruebas de Mutagenicidad , Oxidación-Reducción , Estrés Oxidativo/efectos de los fármacos , Ratas , Ratas Sprague-DawleyRESUMEN
Oxidative damage of DNA and lipids in normal primary rat hepatocyte cultures and in hepatoma Fao cell-line was induced by ferric nitrilotriacetate (Fe-NTA). DNA oxidation was evidenced by measuring the mutagenic oxidized nucleoside 8-hydroxy-2'-deoxyguanosine (8-oxodG). An increase in 8-oxodG production was induced by Fe-NTA in the two different cell cultures. Moreover, this increase was more important in hepatocytes than in Fao cells. In addition, the extent of lipid peroxidation was higher in normal hepatocytes than in Fao cells. These observations demonstrated a higher resistance of tumor cells than normal hepatocytes to oxidative stress. Since DNA lesions induced by oxidative stress are now recognized as being involved in the mutagenesis process and since normal hepatocytes appeared particularly sensitive to iron-induced oxidative damage, a high level of iron should be considered as a potent toxic factor involved in normal cell degeneration. This findings might partly explain the propensity of hepatic iron-overload diseases for cancerous evolution.
Asunto(s)
Daño del ADN/fisiología , Hepatocitos/metabolismo , Peroxidación de Lípido/fisiología , Neoplasias Hepáticas Experimentales/metabolismo , Estrés Oxidativo/fisiología , 8-Hidroxi-2'-Desoxicoguanosina , Animales , Carcinógenos/toxicidad , Línea Celular Tumoral , Transformación Celular Neoplásica/metabolismo , Transformación Celular Neoplásica/patología , Células Cultivadas , Cromatografía Líquida de Alta Presión , Desoxiguanosina/análogos & derivados , Desoxiguanosina/análisis , Desoxiguanosina/metabolismo , Compuestos Férricos/toxicidad , Hepatocitos/efectos de los fármacos , Hepatocitos/patología , Neoplasias Hepáticas Experimentales/patología , Masculino , Ácido Nitrilotriacético/análogos & derivados , Ácido Nitrilotriacético/toxicidad , Ratas , Ratas Sprague-DawleyRESUMEN
Supplementation of rat hepatocyte cultures with the flavonoid myricetin (300 microM) led to the formation of phenoxyl radical intermediates, as detected in intact cells by electron paramagnetic resonance (EPR) spectroscopy. These radicals corresponded to one-electron oxidation products of myricetin. The level of phenoxyl radicals was significantly reduced when myricetin-treated hepatocyte cultures were also supplemented with iron (Fe-NTA 100 microM). This suggested that iron could accelerate the oxidation flux of myricetin. Moreover, myricetin was found to be able to inhibit lipid peroxidation induced by iron in hepatocyte culture. Free malondialdehyde (MDA) levels and the amount of radicals derived from oxidized lipids were greatly reduced when myricetin was added to iron-treated cultures. This showed that myricetin was a good inhibitor of lipid peroxidation in this model and that the intermediate generation of phenoxyl radicals might contribute to the antioxidant mechanism of myricetin.
Asunto(s)
Compuestos Férricos/farmacología , Flavonoides/farmacología , Hierro/farmacología , Peroxidación de Lípido/fisiología , Hígado/efectos de los fármacos , Ácido Nitrilotriacético/análogos & derivados , Fenoles/metabolismo , Animales , Células Cultivadas , Espectroscopía de Resonancia por Spin del Electrón , Radicales Libres/metabolismo , Cinética , Peroxidación de Lípido/efectos de los fármacos , Hígado/metabolismo , Malondialdehído/análisis , Ácido Nitrilotriacético/farmacología , Oxidación-Reducción , RatasAsunto(s)
Reparación del ADN , ADN/efectos de los fármacos , Flavonoides/farmacología , Hepatocitos/efectos de los fármacos , Animales , Northern Blotting , ADN Polimerasa beta/genética , Femenino , Hepatocitos/metabolismo , Masculino , Oxidación-Reducción , ARN Mensajero/genética , Ratas , Ratas Sprague-DawleyRESUMEN
Iron-overload diseases frequently develop hepatocellular carcinoma. The genotoxic mechanism whereby iron is involved in hepatocarcinogenesis might involve an oxidative process via the intermediate production of reactive oxygen species. This was presently investigated by examining kinetics of formation and repair of DNA base lesions in primary rat hepatocyte cultures supplemented with the iron chelate, ferric nitrilotriacetate Fe-NTA (10 and 100 microM). Seven DNA base oxidation products have been identified in DNA extracts by gas chromatography-mass spectrometry, which showed a predominance of oxidized-purines (8-oxo-guanine, xanthine, fapy-adenine, 2-oxo-adenine) above oxidized pyrimidines (5-OHMe-uracil, 5-OH-uracil, 5-OH-cytosine) in control cultures. All these DNA oxidation products revealed a significant dose-dependent increase at 4 to 48 h after Fe-NTA supplementation, among which fapy-adenine showed the highest increase and 5-OH-cytosine was the least prominent. Involvement of iron in this oxidative process was established by a correlation between extent in DNA oxidation and intracellular level of toxic low molecular weight iron. DNA excision-repair activity was estimated by release of DNA oxidation products in culture medium. All the seven DNA oxidation products were detected in the medium of control cultures and showed basal repair activity. This DNA repair activity was increased in a time- and dose-dependent fashion with Fe-NTA. Oxidized-pyrimidines, among which was 5-OHMe-Uracil, were preferentially repaired, which explains the low levels detected in oxidized DNA. Since oxidized bases substantially differed from one another in terms of excision rates from cellular DNA, specific excision-repair enzymes might be involved. Our findings, however, demonstrate that even though DNA repair pathways were activated in iron-loaded hepatocyte cultures, these processes were not stimulated enough to prevent an accumulation of highly mutagenic DNA oxidative products in genomic DNA. The resulting genotoxic effect of Fe-NTA might be relevant in understanding the hepatocarcinogenic evolution of iron-overload diseases.