RESUMEN
A series of 2-piperazin-1-yl-quinazolines were synthesized and evaluated for their antiaggregative activity. The synthesized small molecule compounds have potently inhibited platelet aggregation in vitro and blocked FITC-Fg binding to αIIbß3 integrin in a suspension of washed human platelets. The key αIIbß3 protein-ligand interactions were determined in docking experiments and some correlations have been observed between values of the affinity and docking scores.
Asunto(s)
Plaquetas/efectos de los fármacos , Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/metabolismo , Quinazolinas/química , Quinazolinas/farmacología , Plaquetas/citología , Plaquetas/metabolismo , Humanos , Ligandos , Simulación del Acoplamiento Molecular , Piperazinas/química , Piperazinas/farmacología , Agregación Plaquetaria/efectos de los fármacosRESUMEN
A series of novel RGD mimetics containing phthalimidine fragment was designed and synthesized. Their antiaggregative activity determined by Born's method was shown to be due to inhibition of fibrinogen binding to αIIbß3. Molecular docking of RGD mimetics to αIIbß3 receptor showed the key interactions in this complex, and also some correlations have been observed between values of biological activity and docking scores. The single crystal X-ray data were obtained for five mimetics.
Asunto(s)
Materiales Biomiméticos/química , Isoindoles/química , Oligopéptidos/química , Ftalimidas/química , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/química , Sitios de Unión , Materiales Biomiméticos/metabolismo , Materiales Biomiméticos/farmacología , Cristalografía por Rayos X , Fibrinógeno/antagonistas & inhibidores , Fibrinógeno/metabolismo , Humanos , Isoindoles/metabolismo , Isoindoles/farmacología , Ligandos , Modelos Moleculares , Simulación del Acoplamiento Molecular , Estructura Molecular , Oligopéptidos/metabolismo , Oligopéptidos/farmacología , Ftalimidas/metabolismo , Ftalimidas/farmacología , Agregación Plaquetaria/efectos de los fármacos , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/antagonistas & inhibidores , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/metabolismo , Unión ProteicaRESUMEN
The novel RGD mimetics with phthalimidine central fragment were synthesized with the use of 4-piperidine-4-yl-butyric, 4-piperidine-4-yl-benzoic, 4-piperazine-4-yl-benzoic and 1,2,3,4-tetrahydroisoquinoline-7-carboxylic acids as surrogates of Arg motif. The synthesized compounds potently inhibited platelet aggregation in vitro and blocked FITC-Fg binding to α(IIb)ß(3) integrin in a suspension of washed human platelets. The key α(IIb)ß(3) protein-ligand interactions were determined in docking experiments.
Asunto(s)
Diseño de Fármacos , Ftalimidas/síntesis química , Inhibidores de Agregación Plaquetaria/síntesis química , Inhibidores de Agregación Plaquetaria/farmacología , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/antagonistas & inhibidores , Receptores Fibrinógenos/antagonistas & inhibidores , Arginina/análogos & derivados , Arginina/metabolismo , Plaquetas/metabolismo , Evaluación Preclínica de Medicamentos , Fibrinógeno/metabolismo , Fluoresceína-5-Isotiocianato/metabolismo , Humanos , Isoquinolinas/química , Isoquinolinas/metabolismo , Ligandos , Oligopéptidos/química , Oligopéptidos/metabolismo , Inhibidores de Agregación Plaquetaria/química , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/metabolismo , Unión Proteica , Receptores Fibrinógenos/metabolismo , Programas Informáticos , Estereoisomerismo , Relación Estructura-Actividad , Tirofibán , Tirosina/análogos & derivados , Tirosina/química , Tirosina/metabolismoRESUMEN
The novel RGDF mimetics were synthesized with the use of 4-(1,2,3,4-tetrahydroisoquinoline-7-yl)amino-4-oxobutyric or 5-(1,2,3,4-tetrahydroisoquinoline-7-yl)amino-5-oxopentanoic acids as a surrogate of Arg-Gly motif. The synthesized compounds have demonstrated a high potency to inhibit platelet aggregation in vitro and to block FITC-Fg binding to alpha(IIb)beta(3) on washed human platelets.
Asunto(s)
Inhibidores de Agregación Plaquetaria/síntesis química , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/antagonistas & inhibidores , Tetrahidroisoquinolinas/química , Fibrinógeno/química , Fluoresceína-5-Isotiocianato/química , Humanos , Oligopéptidos/química , Agregación Plaquetaria/efectos de los fármacos , Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/metabolismo , Unión Proteica , Tetrahidroisoquinolinas/síntesis química , Tetrahidroisoquinolinas/farmacologíaRESUMEN
It has been proposed a novel method for obtaining of 1,2,3,4-tetrahydroisoquinoline-7-carboxylic acid as Arg-mimetic within the framework of search for novel fibrinogen receptor antagonists. New alpha (IIb)beta(3) antagonists were prepared on a base of 1,2,3,4-tetrahydroisoquinoline-7-carboxylic acid. Their high antiaggregatory activities in a human platelet rich plasma and ability to block FITC-Fg binding to alpha (IIb)beta(3) on washed human platelets were estimated.
Asunto(s)
Receptores Fibrinógenos/antagonistas & inhibidores , Tetrahidroisoquinolinas/síntesis química , Tetrahidroisoquinolinas/farmacología , Plaquetas/efectos de los fármacos , Plaquetas/metabolismo , Fibrinógeno/metabolismo , Humanos , Espectroscopía de Resonancia Magnética , Estructura Molecular , Oligopéptidos/antagonistas & inhibidores , Agregación Plaquetaria/efectos de los fármacos , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/metabolismo , Plasma Rico en Plaquetas/efectos de los fármacos , Unión Proteica/efectos de los fármacos , Receptores Fibrinógenos/metabolismo , Tetrahidroisoquinolinas/químicaRESUMEN
Two novel RGDF mimetics were synthesized with the use of 4-(1,2,3,4-tetrahydro-isoquinoline-7-yl)amino-4-oxo-butyric acid as a new surrogate of Arg-Gly motif. The synthesized compounds have demonstrated a high potency to inhibit platelet aggregation in vitro and to block FITC-Fg binding to alphaIIbbeta3 on washed human platelets.
Asunto(s)
Receptores Fibrinógenos/antagonistas & inhibidores , Isoquinolinas , Espectroscopía de Resonancia Magnética , Espectrometría de Masa Bombardeada por Átomos VelocesRESUMEN
This article describes design, virtual screening, synthesis, and biological tests of novel αIIbß3 antagonists, which inhibit platelet aggregation. Two types of αIIbß3 antagonists were developed: those binding either closed or open form of the protein. At the first step, available experimental data were used to build QSAR models and ligand- and structure-based pharmacophore models and to select the most appropriate tool for ligand-to-protein docking. Virtual screening of publicly available databases (BioinfoDB, ZINC, Enamine data sets) with developed models resulted in no hits. Therefore, small focused libraries for two types of ligands were prepared on the basis of pharmacophore models. Their screening resulted in four potential ligands for open form of αIIbß3 and four ligands for its closed form followed by their synthesis and in vitro tests. Experimental measurements of affinity for αIIbß3 and ability to inhibit ADP-induced platelet aggregation (IC50) showed that two designed ligands for the open form 4c and 4d (IC50 = 6.2 nM and 25 nM, respectively) and one for the closed form 12b (IC50 = 11 nM) were more potent than commercial antithrombotic Tirofiban (IC50 = 32 nM).
Asunto(s)
Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/antagonistas & inhibidores , Relación Estructura-Actividad Cuantitativa , Técnicas de Química Sintética , Diseño de Fármacos , Evaluación Preclínica de Medicamentos/métodos , Humanos , Concentración 50 Inhibidora , Modelos Moleculares , Simulación del Acoplamiento Molecular , Oligopéptidos/química , Peptidomiméticos/química , Peptidomiméticos/farmacología , Inhibidores de Agregación Plaquetaria/síntesis química , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/química , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/metabolismo , Bibliotecas de Moléculas Pequeñas/química , Bibliotecas de Moléculas Pequeñas/farmacologíaRESUMEN
A scheme of synthesis of previously obtained RGDF-peptidomimetic-4-oxo-4-(piperazine-1-yl)butyrylglycyl-D,L-beta-phenyl-beta-alanine (I), was simplified. The novel RGDF-peptidomimetic -4-oxo-4-piperazine-1-yl)butyryl-glycyl-L-aspartyl-L-phenylalanine (II) was synthesized with the use of 4-oxo-4-(piperazine-1-yl)butyric acid as arginyl mimetic. The obtained pseudopeptides were able to inhibit both platelet aggregation in human blood and binding of fibrinogen to its receptor.
Asunto(s)
Oligopéptidos/química , Oligopéptidos/síntesis química , Piperazinas/síntesis química , Inhibidores de Agregación Plaquetaria/síntesis química , Plaquetas/metabolismo , Química Farmacéutica , Fibrinógeno/metabolismo , Humanos , Técnicas In Vitro , Imitación Molecular , Oligopéptidos/farmacología , Piperazinas/química , Piperazinas/farmacología , Inhibidores de Agregación Plaquetaria/química , Inhibidores de Agregación Plaquetaria/farmacología , Unión ProteicaRESUMEN
The novel fibrinogen receptor antagonists containing fragments of 7-amino-1,2,3,4-tetrahydroisoquinoline and isophthalic acids were synthesized and successfully tested for their ability to inhibit platelet aggregation in vitro and to block FITC-Fg binding to alpha(IIb)beta(3) on washed human platelets.
Asunto(s)
Ácidos Ftálicos/farmacología , Inhibidores de Agregación Plaquetaria/farmacología , Agregación Plaquetaria/efectos de los fármacos , Receptores Fibrinógenos/antagonistas & inhibidores , Unión Competitiva/efectos de los fármacos , Bioensayo , Evaluación Preclínica de Medicamentos , Humanos , Imitación Molecular , Estructura Molecular , Ácidos Ftálicos/síntesis química , Ácidos Ftálicos/química , Inhibidores de Agregación Plaquetaria/síntesis química , Inhibidores de Agregación Plaquetaria/química , Complejo GPIIb-IIIa de Glicoproteína Plaquetaria/efectos de los fármacos , Unión Proteica/efectos de los fármacos , Estereoisomerismo , Relación Estructura-ActividadRESUMEN
The novel RGDF mimetics 9a and 9b were synthesized with the use of 4-(isoindoline-5-yl)amino-4-oxobutyric acid as a surrogate of Arg-Gly motif. The synthesized compounds have demonstrated a high potency to inhibit platelet aggregation in vitro and to block FITC-Fg binding to alpha(IIb)beta(3) on washed human platelets.