RESUMEN
Two new cases that conform to the clinical and histopathological features of recurring digital fibrous tumor of childhood, described by Reye in 1965, are reported. This tumor, which is considered a distinct entity among the juvenile fibromatoses, characteristically presents in infancy and early childhood, involves only the digits, recurs frequently following surgical excision without metastatic spread, and demonstrates the distinct histopathological finding of intracytoplasmic inclusion bodies within proliferated fibroblasts. This tumor is reviewed with reference to clinical features, histopathology, etiological considerations, and management.
Asunto(s)
Fibroma , Dedos , Enfermedades del Pie , Dedos del Pie , Adolescente , Femenino , Fibroma/patología , Fibroma/cirugía , Dedos/patología , Dedos/cirugía , Enfermedades del Pie/etiología , Enfermedades del Pie/patología , Enfermedades del Pie/cirugía , Humanos , Lactante , Recurrencia Local de Neoplasia , Dedos del Pie/patología , Dedos del Pie/cirugíaRESUMEN
An indirect immunoperoxidase staining method has been successfully applied to 4% formaldehyde solution tissue sections fixed in and embedded in paraffin for the localization of Sporothrix schenckil and Cryptococcus neoformans without prior trypsinization of tissue sections. A comparison of this method with an analogous immunofluorescence staining technique has been made.
Asunto(s)
Cryptococcus neoformans/aislamiento & purificación , Cryptococcus/aislamiento & purificación , Técnicas para Inmunoenzimas , Sporothrix/aislamiento & purificación , Coloración y Etiquetado/métodos , Técnica del Anticuerpo Fluorescente , Formaldehído , Humanos , Parafina , Piel/inmunologíaRESUMEN
The spirochete, Treponema pallidum, the causative agent of syphilis, has been successfully localized in formaldehydefixed and paraffin-embedded tissue sections using rabbit anti-T pallidum antiserum with two immunoperoxidase techniques. These techniques, the indirect peroxidase-labeled antibody method and the peroxidase-antiperoxidase (PAP) method are compared for sensitivity and degree of nonspecific staining. Both offer substantial advantages over conventional silver-impregnation techniques, but the indirect peroxidase-labeled antibody method seems better, based on the intensity of staining and the simplicity of procedure.