Mapping of functional brain activity in freely behaving rats during voluntary running using manganese-enhanced MRI: implication for longitudinal studies.

Magnetic resonance imaging (MRI) is widely used in basic and clinical research to map the structural and functional organization of the brain. An important need of MR research is for contrast agents that improve soft-tissue contrast, enable visualization of neuronal tracks, and enhance the capacity of MRI to provide functional information at different temporal scales. Unchelated manganese can be such an agent, and manganese-enhanced MRI (MEMRI) can potentially be an excellent technique for localization of brain activity (for review see Silva et al., 2004). Yet, the toxicity of manganese presents a major limitation for employing MEMRI in behavioral paradigms. We have tested systematically the voluntary wheel running behavior of rats after systemic application of MnCl(2) in a dose range of 16-80 mg/kg, which is commonly used in MEMRI studies. The results show a robust dose-dependent decrease in motor performance, which was accompanied by weight loss and decrease in food intake. The adverse effects lasted for up to 7 post-injection days. The lowest dose of MnCl(2) (16 mg/kg) produced minimal adverse effects, but was not sufficient for functional mapping. We have therefore evaluated an alternative method of manganese delivery via osmotic pumps, which provide a continuous and slow release of manganese. In contrast to a single systemic injection, the pump method did not produce any adverse locomotor effects, while achieving a cumulative concentration of manganese (80 mg/kg) sufficient for functional mapping. Thus, MEMRI with such an optimized manganese delivery that avoids toxic effects can be safely applied for longitudinal studies in behaving animals.

An investigation of factors affecting test equating in latent trait theory.

The study investigated five factors which can affect the equating of scores from two tests onto a common score scale. The five factors were: (a) item distribution type (i.e., normal versus uniform; (b) standard deviation of item difficulty (i.e.,.68,.95,.99); (c) number of items or test length (i.e., 50, 100, 200); (d) number of common items (i.e., 10, 20, 30); and (e) sample size (i.e., 100, 300, 500). SIMTEST and BIGSTEPS programs were used for the simulation and equating of 4,860 item data sets, respectively. Results from the five-way fixed effects factorial analysis of variance indicated three statistically significant two-way interaction effects. Simple effects for the interaction between common item length and test length only were interpreted given Type I error rate considerations. The eta-squared values for number of common items and test length were small indicating the effects had little practical importance. The Rasch approach to equating is robust with as few as 10 common items and a test length of 100 items.

Magnetic resonance imaging of cortical connectivity in vivo.

Magnetic resonance imaging of neuronal connectivity in vivo opens up the possibility of performing longitudinal investigations on neuronal networks. This is one main reason for the attention that paramagnetic ion manganese (Mn2+) has attracted as a potential anterograde neuronal tracer for MRI experiments. However, the correct and possibly repeated use of this tracer--or of any tracer for that matter, including heavy metals--requires the development of an administration strategy that minimizes its toxic effects. Here we first investigated the conditions that maximize the tracing efficiency of Mn2+ and preserve viability and tissue architectonics in combined MRI and histology experiments in rats. We demonstrate that most common protocols for neuronal tract tracing using Mn2+ result in large neuronal and glial lesions. The toxicity of manganese is distinct during intracortical injections and blocks the transfer of the tracer. After optimizing the technique, we could show that extensive cortical connectivity maps can be generated, with no sign of neuronal damage. Importantly, preservation of tissue viability improves the efficiency of Mn2+ in tracing neuronal connections. We have successfully used this technique to trace corticofugal somatosensory and motor pathways in individual animals and describe a connectivity index (CnI) based on Mn2+ transport that quantitatively reveals cortical heterogeneities in interhemispheric communication. Finally, we have significantly improved the resolution of the technique by continuously infusing very low concentrations of Mn2+ into the target area using osmotic pumps coupled to chronically implanted brain cannulae. The specific, nontoxic and quantitative nature of the neuronal tracings described here indicates the value of this tracer for chronic studies of development and plasticity as well as for studies of brain pathology.

Design and fabrication of computer-generated beam-shaping holograms.

For the design of computer-generated holograms reconstructing certain intensity patterns with phase freedom, we use an object-oriented approach. The given intensity pattern is decomposed into elementary objects for which appropriate phase-only hologram functions can be constructed. The total hologram function is found by the subsequent superposition of its constituents, with a relative amplitude and phase weighting for each of them. Thus, the degrees of freedom are dramatically reduced compared with those of sampling approaches. The design algorithm allows us to compensate on the one hand for the intensity and phase distribution of the impinging laser beam and on the other hand for the shape of the hologram aperture. We report on the computer-aided design of such holograms, as well as their fabrication through the use of laser lithography and reactive ion etching. Optical reconstructions are shown.

Design of computer-generated beam-shaping holograms by iterative finite-element mesh adaption.

Computer-generated phase-only holograms can be used for laser beam shaping, i.e., for focusing a given aperture with intensity and phase distributions into a pregiven intensity pattern in their focal planes. A numerical approach based on iterative finite-element mesh adaption permits the design of appropriate phase functions for the task of focusing into two-dimensional reconstruction patterns. Both the hologram aperture and the reconstruction pattern are covered by mesh mappings. An iterative procedure delivers meshes with intensities equally distributed over the constituting elements. This design algorithm adds new elementary focuser functions to what we call object-oriented hologram design. Some design examples are discussed.

Assessing anxiety about retirement: the case of academicians.

One hundred and forty-four individuals, ninety-two of whom were active university faculty and fifty-two of whom were retired, completed a measure of anxiety about retirement as well as a battery of self-report scales assessing a number of personality, job-related, and retirement-specific constructs. Results suggested that the newly developed measure of retirement anxiety possessed high internal consistency as well as both discriminant and construct validity. Moreover, it is multidimensional in nature, and distinct in content from previous scales assessing anxiety about retirement. Its use for persons anticipating retirement is discussed in the context of the benefits of a proactive stance toward retirement preparation.

Employee age and perceptions of work in self-managing and traditional work groups.

Self-managing work groups are a form of work design in which employees take responsibility for the group's tasks and have discretion over decisions which impact group performance. To explore the impact of age and work teams on job attitudes, data from 477 employees suggested that self-managed work group members differed from traditional job holders regarding perceived general job satisfaction, perceived control by supervisors, as well as a number of specific dimensions of the work environment. Moreover, while there was evidence of an age effect on attitudes toward supervisory control, there was no joint effect of age by work design on job attitudes, i.e., one's perceived general job satisfaction. Older employees who were members of self-managed work groups were however, more impacted by this form of work design in reporting more positive perceptions of their access to information essential to the performance of their work. These findings suggest that an "older" work force should not be considered a barrier to implementing a work teams approach to job design.