Asunto(s)
Lesión Pulmonar Aguda/inducido químicamente , Factor Estimulante de Colonias de Granulocitos/efectos adversos , Movilización de Célula Madre Hematopoyética/efectos adversos , Enfermedades Pulmonares Intersticiales/etiología , Trasplante de Células Madre de Sangre Periférica , Donante no Emparentado , Lesión Renal Aguda/inducido químicamente , Lesión Pulmonar Aguda/etiología , Adulto , Bronquitis/complicaciones , Enterococcus faecium , Femenino , Infecciones por Bacterias Grampositivas/complicaciones , Infecciones por Bacterias Grampositivas/microbiología , Factor Estimulante de Colonias de Granulocitos/farmacología , Humanos , Subtipo H1N1 del Virus de la Influenza A/aislamiento & purificación , Virus de la Influenza B/aislamiento & purificación , Gripe Humana/complicaciones , Gripe Humana/virología , Lenograstim , Enfermedades Pulmonares Intersticiales/tratamiento farmacológico , Masculino , Otitis Media/complicaciones , Otitis Media/microbiología , Derrame Pleural/etiología , Neumonía Viral/complicaciones , Neumonía Viral/virología , Prednisona/uso terapéutico , Proteínas Recombinantes/efectos adversos , Proteínas Recombinantes/farmacología , Adulto JovenRESUMEN
The developmental kinetics of the inner ear of 2- to 30-mm embryos are examined. The development of the inner ear can be shown as being constructively correlated with the development of the brain and thus being an ontogenic requisite for the structural development of the embryo. The development kinetic findings, as shown here, demonstrate that the functions of the inner ear have already started during its development and differentiation.
Asunto(s)
Oído Interno/embriología , Vías Auditivas/embriología , Encéfalo/embriología , Diferenciación Celular , Cóclea/embriología , Dendritas/ultraestructura , Oído Interno/citología , Oído Interno/inervación , Conducto Endolinfático/embriología , Femenino , Edad Gestacional , Humanos , Cinética , Embarazo , Sáculo y Utrículo/embriologíaRESUMEN
LCM virus, strain WE--grown on L cells--and labeled with 3H-uridine was centrifuged to equilibrium in a sucrose density gradient and examined in fractions for infectivity, incorporated radioactivity, and electron-microscopic features. The peak of infectivity is congruent with the one of radioactivity (density = 1.17 g/ml). LCM virus specificity of the radioactive peak was proved by precipitation of the radioactivity with anti-LCM virus antiserum. The peak fractions showed an abundance of 106 +/- 14 nm (1s) particles. They could be agglutinated with specific anti-LCM virus antiserum but not with antiserum directed against the histocompatibility (H-2) antigens of L cells.
Asunto(s)
Virus de la Coriomeningitis Linfocítica/aislamiento & purificación , Epítopos , Células L , Virus de la Coriomeningitis Linfocítica/inmunología , Virus de la Coriomeningitis Linfocítica/ultraestructura , Microscopía ElectrónicaRESUMEN
A specific and sensitive solid-phase radioimmunoassay has been developed for the detection of LCM virus antigens and anti-LCM virus antibodies. The test was performed in a microtiter system using polyvinylcholoride wells coated with anti-LCM virus rabbit hyperimmune serum. LCM virus antigens were allowed to bind to this antibody and afterwards detected by 125J-labeled anti-LCM virus-gamma-globulin. Anti-LCM virus antibodies were assayed by specific inhibition of these bound antigens. Since this technique is rapid and easy to perform the solid-phase radioimmunoassay is a valuable test for detecting LCM virus infections. Selected at random, 208 girls with, and 208 girls without, contact to Syrian hamsters were investigated for anti-LCM virus antibodies. Five (2.4%) of the hamster-exposed girls had anti-LCM virus antibodies (RIA-titer 1:8-1:32) in contrast to none of the control group (P=0.03).
Asunto(s)
Anticuerpos Antivirales/análisis , Antígenos Virales/análisis , Encuestas Epidemiológicas , Virus de la Coriomeningitis Linfocítica/inmunología , Animales , Especificidad de Anticuerpos , Sitios de Unión de Anticuerpos , Cricetinae , Femenino , Humanos , Coriomeningitis Linfocítica/epidemiología , Mesocricetus , RadioinmunoensayoRESUMEN
The correlation between lung function and the quality of oesophageal speech was investigated in 36 patients who had undergone laryngectomy with glottoplasty. It was found that this correlation did not depend on any important lung function parameters. Lung tests were carried out on 10 patients before and after laryngectomy. No changes in lung function could be found that may have been directly attributed to a laryngectomy. This means that restrictive or obstructive lung disturbances do not contra-indicate glottoplasty or a Blom-Singer-puncture.