RESUMEN
Phylogenetic analyses of sequences generated from portions of three genes coding for the proteins enolase (enoA), beta-tubulin (benA), and calmodulin (calM) of a large number of isolates within the section Terrei, genus Aspergillus, revealed the presence of a new cryptic species within this section, Aspergillus alabamensis. Most members of this new cryptic species were recovered as colonizing isolates from immunocompetent patient populations, had decreased in vitro susceptibilities to the antifungal drug amphotericin B, and were morphologically similar to but genetically distinct from Aspergillus terreus isolates.
Asunto(s)
Aspergilosis/microbiología , Aspergillus/clasificación , Aspergillus/aislamiento & purificación , Aspergillus/genética , Aspergillus/metabolismo , Calmodulina/genética , Proteínas Fúngicas/genética , Humanos , Datos de Secuencia Molecular , Técnicas de Tipificación Micológica , Fosfopiruvato Hidratasa/genética , Filogenia , Tubulina (Proteína)/genéticaRESUMEN
We evaluated the susceptibility of 85 Fusarium spp. isolates from cases of fungal keratitis with 8 antifungal drugs using the standard Clinical and Laboratory Standards Institute broth microdilution and E test methods. Members of the Fusarium solani species complex showed consistently higher MICs to the triazole drugs itraconazole, voriconazole, and posaconazole than did members of other species complexes (Fusarium oxysporum and other minor species). High MICs to amphotericin B, natamycin, and echinocandins were consistently obtained with no discrimination based on species or method. Further work is required to determine any potential correlation between MIC and clinical outcome in keratitis.