RESUMEN
Resistance to acetylsalicylic acid (ASA) in patients with coronary artery disease is a poor predictor for the development of atherothrombotic complications. In 277 patients with coronary artery disease suffered uncomplicated coronary angioplasty with stent implantation, we was estimated arachidon-induced platelet aggregation during treatment with acetylsalicylic acid by bedside VerifyNow Assay test at 28-90 days after the intervention. It was found that 18.9% of the 144 patients receiving a combination of ASA 75 mg with 15.2 mg of magnesium hydroxide had true (laboratory) resistance to ASA. At the same time on the original enteric coated ASA 100 mg, we can found only 0.8% resistance to ASA among 129 patients. We made switch from combination of ASA 75 mg with 15.2 mg of magnesium hydroxide to original enteric coated ASA 100 mg and repeat VerifyNow Assay test at 2-4 days and found lost of resistance in 92% of 28 patients. Thus, resistance to the ASA is not constant, it depends on the form and the applied dose of ASA, and eliminating more than 92% when ASA changes from ineffective to effective form.
Asunto(s)
Aspirina/farmacología , Enfermedad de la Arteria Coronaria , Inhibidores de Agregación Plaquetaria/farmacología , Anciano , Anciano de 80 o más Años , Angioplastia Coronaria con Balón , Aspirina/uso terapéutico , Enfermedad de la Arteria Coronaria/tratamiento farmacológico , Enfermedad de la Arteria Coronaria/cirugía , Resistencia a Medicamentos , Femenino , Humanos , Masculino , Persona de Mediana Edad , Agregación Plaquetaria/efectos de los fármacos , Inhibidores de Agregación Plaquetaria/uso terapéutico , StentsRESUMEN
The human chromosome (HSA)19q region has been shown to correspond to swine chromosome (SSC) 6q11-->q21 by bi-directional chromosomal painting and gene mapping. However, since the precise correspondence has not been determined, 26 genes localized in HSA19q13.3-->q13.4 were assigned to the SSC6 region mainly by radiation hybrid (RH) mapping, and additionally, by somatic cell hybrid panel (SCHP) mapping, and fluorescent in situ hybridization (FISH). Out of the 26 genes, 24 were assigned to a swine RH map with LOD scores greater than 6 (threshold of significance). The most likely order of the 24 genes along SSC6 was calculated by CarthaGene, revealing that the order is essentially the same as that in HSA19q13.3-->q13.4. For AURKC and RPS5 giving LOD scores not greater than 6, SCHP mapping and FISH were additionally performed; SCHP mapping assigned AURKC and RPS5 to SSC6q22-->q23 and SSC6q21, respectively, which is consistent with the observation of FISH. Consequently, all the genes (26 genes) examined in the present study were shown to localize in SSC6q12-->q23, and the order of the genes along the chromosomes was shown to be essentially the same in swine and human, though several intrachromosomal rearrangements were observed between the species.
Asunto(s)
Cromosomas Humanos Par 19/genética , Cromosomas de los Mamíferos/genética , Orden Génico/genética , Mapeo de Híbrido por Radiación/métodos , Homología de Secuencia de Ácido Nucleico , Porcinos/genética , Animales , Cartilla de ADN/genética , Genes/genética , Genoma , Genoma Humano , HumanosRESUMEN
A comprehensive and comparative map was constructed for the porcine chromosome (SSC) 6q11-->q21 region, where the gene(s) responsible for the maldevelopment of embryos are localized using swine populations of the National Institute of Animal Industry, Japan (NIAI). Since the chromosomal region corresponds to a region of human chromosome (HSA) 19q13.1-->q13.3 based on bi-directional chromosome painting, primer pairs were designed from porcine cDNA sequences identified, on a sequence comparison basis, as being transcripts from genes orthologous to those in the HSA region. Fifty-one genes were successfully assigned to a swine radiation hybrid (RH) map with LOD scores greater than 6. ERF and PSMD8 genes were assigned to SSC4 and SSC1, respectively. The remaining 49 genes were assigned to SSC6, demonstrating that the synteny between the SSC6 and HSA19 chromosomal regions is essentially conserved, therefore confirming, the results of bi-directional chromosome painting. However, when examined precisely, rearrangements have apparently occurred within the region of conserved synteny. For the ERF and PSMD8 genes assigned to SSCs other than SSC6, additional mapping using somatic cell hybrid (SCH) panels was performed to confirm the results of RH-mapping.
Asunto(s)
Cromosomas Humanos Par 19/genética , Cromosomas/genética , Genes/genética , Mapeo de Híbrido por Radiación/métodos , Mapeo de Híbrido por Radiación/veterinaria , Porcinos/genética , Animales , Secuencia Conservada/genética , Cricetinae , Cricetulus/genética , Cartilla de ADN/genética , Etiquetas de Secuencia Expresada , Orden Génico/genética , Marcadores Genéticos/genética , Genoma , Humanos , Mapeo Físico de Cromosoma/métodos , Mapeo Físico de Cromosoma/veterinariaRESUMEN
The localization of the putative testis determining gene (TDF) was established with isotopic and nonisotopic methods of in situ hybridization in prometaphasic chromosomes of cattle. The results of both the methods were seen to coincide. The sites of hybridization have been revealed in X-chromosome (R-bands, q2.1 and q2.3.1). TDF was also localized in the proximal part of the long Y-chromosome arm.
Asunto(s)
Bovinos/genética , Mapeo Cromosómico , Genes/genética , Testículo/embriología , Cromosoma X/ultraestructura , Cromosoma Y/ultraestructura , Animales , Mapeo Cromosómico/métodos , ADN/genética , Sondas de ADN , Linfocitos/ultraestructura , Masculino , Metafase , Hibridación de Ácido Nucleico , TritioRESUMEN
The uniform distribution of satellite DNA II and IV has been revealed using in situ hybridization and differential staining in centromeric regions of autosomes. The sex chromosomes have not found such nucleotide blocks. There is only minor satellite IV block inside Y chromosome short arm. The Y chromosome has got some (TG)n enriched blocks distributed also among other parts of genome and one copy of sequences like human ZFY gene. The high repetitive fraction of bovine genomic DNA have not revealed RFLP. However, the difference has been found by blot hybridization between genomic organization of satellite IV in cattle and yak chromosomal DNA. Non-Mendelian distribution of some such nucleotide blocks has been obtained for interspecies crosses of cattle and yak.