Gray matter textural heterogeneity as a potential in-vivo biomarker of fine structural abnormalities in Asperger syndrome.

Brain imaging studies contribute to the neurobiological understanding of Autism Spectrum Conditions (ASC). Herein, we tested the prediction that distributed neurodevelopmental abnormalities in brain development impact on the homogeneity of brain tissue measured using texture analysis (TA; a morphological method for surface pattern characterization). TA was applied to structural magnetic resonance brain scans of 54 adult participants (24 with Asperger syndrome (AS) and 30 controls). Measures of mean gray-level intensity, entropy and uniformity were extracted from gray matter images at fine, medium and coarse textures. Comparisons between AS and controls identified higher entropy and lower uniformity across textures in the AS group. Data reduction of texture parameters revealed three orthogonal principal components. These were used as regressors-of-interest in a voxel-based morphometry analysis that explored the relationship between surface texture variations and regional gray matter volume. Across the AS but not control group, measures of entropy and uniformity were related to the volume of the caudate nuclei, whereas mean gray-level was related to the size of the cerebellar vermis. Similar to neuropathological studies, our study provides evidence for distributed abnormalities in the structural integrity of gray matter in adults with ASC, in particular within corticostriatal and corticocerebellar networks. Additionally, this in-vivo technique may be more sensitive to fine microstructural organization than other more traditional magnetic resonance approaches and serves as a future testable biomarker in AS and other neurodevelopmental disorders.

Membrane organization of photosystem I complexes in the most abundant phototroph on Earth.

Prochlorococcus is a major contributor to primary production, and globally the most abundant photosynthetic genus of picocyanobacteria because it can adapt to highly stratified low-nutrient conditions that are characteristic of the surface ocean. Here, we examine the structural adaptations of the photosynthetic thylakoid membrane that enable different Prochlorococcus ecotypes to occupy high-light, low-light and nutrient-poor ecological niches. We used atomic force microscopy to image the different photosystem I (PSI) membrane architectures of the MED4 (high-light) Prochlorococcus ecotype grown under high-light and low-light conditions in addition to the MIT9313 (low-light) and SS120 (low-light) Prochlorococcus ecotypes grown under low-light conditions. Mass spectrometry quantified the relative abundance of PSI, photosystem II (PSII) and cytochrome b6f complexes and the various Pcb proteins in the thylakoid membrane. Atomic force microscopy topographs and structural modelling revealed a series of specialized PSI configurations, each adapted to the environmental niche occupied by a particular ecotype. MED4 PSI domains were loosely packed in the thylakoid membrane, whereas PSI in the low-light MIT9313 is organized into a tightly packed pseudo-hexagonal lattice that maximizes harvesting and trapping of light. There are approximately equal levels of PSI and PSII in MED4 and MIT9313, but nearly twofold more PSII than PSI in SS120, which also has a lower content of cytochrome b6f complexes. SS120 has a different tactic to cope with low-light levels, and SS120 thylakoids contained hundreds of closely packed Pcb-PSI supercomplexes that economize on the extra iron and nitrogen required to assemble PSI-only domains. Thus, the abundance and widespread distribution of Prochlorococcus reflect the strategies that various ecotypes employ for adapting to limitations in light and nutrient levels.

A feasibility study of a rotary planar electrode array for electrical impedance mammography using a digital breast phantom.

A feasibility study of an electrical impedance mammography (EIM) system with a rotary planar electrode array, named RPEIM, is presented. The RPEIM system is an evolution of the Sussex MK4 system, which is a prototype instrument for breast cancer detection. Comparing it with the other planar electrode EIM systems, the rotation feature enables a dramatic increase in the number of independent measurements. To assist impedance evaluation exploiting electrode array rotation, a synchronous mesh method is proposed. Using the synchronous mesh method, the RPEIM system is shown to have superior performance in image accuracy, spatial resolution and noise tolerance over the MK4 system. To validate the study, we report simulations based on a close-to-realistic 3D digital breast phantom, which comprises of: skin, nipple, ducts, acinus, fat and tumor. A digital breast phantom of a real patient is constructed, whose tumor was detected using the MK4 system. The reconstructed conductivity image of the breast phantom indicates that the breast phantom is a close replica of the patient's real breast as assessed by the MK4 system in a clinical trial. A comparison between the RPEIM system and the MK4 system is made based on this phantom to assess the advantages of the RPEIM system.

Low-dose postoperative aprotinin reduces mediastinal drainage and blood product use in patients undergoing primary coronary artery bypass grafting who are taking aspirin: a prospective, randomized, double-blind, placebo-controlled trial.

BACKGROUND: Although low-dose aprotinin administered after cardiopulmonary bypass has been reported to reduce mediastinal blood loss and blood product requirements in patients not taking aspirin, it is unknown whether low-dose postoperative aprotinin has any beneficial effects in patients undergoing coronary artery bypass operations who are at high risk of excessive postoperative bleeding and increased transfusion requirements because of aspirin use until just before the operation. METHODS: Fifty-five patients undergoing primary coronary artery operations with cardiopulmonary bypass who continued taking aspirin (150 mg/d) until the day before the operation were enrolled in a prospective, randomized, double-blind trial to receive a single dose of either placebo (n = 29) or 2 x 10(6) kallikrein inhibiting units of aprotinin (n = 26) at the time of sternal skin closure. RESULTS: Patients in the aprotinin group had a lower rate (28 +/- 18 vs 43 +/- 21 mL/h [mean +/- standard deviation], P <.005) and total volume of mediastinal drainage (955 +/- 615 vs 1570 +/- 955 mL, P <.007), as well as a shorter duration of mediastinal drain tube insertion (24.4 +/- 13.8 vs 31.3 +/- 16.5 hours, P <.05). In addition, a smaller proportion of patients receiving aprotinin required a blood product (31% vs 62%, P =.03), resulting in a reduction in the use of packed cells by 47% (P =.05), platelets by 77% (P =.01), fresh frozen plasma by 88% (P =.03), and total blood products by 68% (P =.01) in this group. CONCLUSIONS: These results suggest that postoperative administration of low-dose aprotinin in patients taking aspirin until just before primary coronary artery operations with cardiopulmonary bypass not only reduces the rate and total amount of postoperative mediastinal blood loss but also lowers postoperative blood product use.

Bioimpedance analysis for the characterization of breast cancer cells in suspension.

The bioimpedance spectroscopy (BIS) technique is potentially a useful tool to differentiate malignancy based on the variation of electrical properties presented by different tissues and cells. The different tissues and cells present variant electrical resistance and reactance when excited at different frequencies. The main purpose of this area of research is to use impedance measurements over a low-frequency bandwidth ranging from 1 kHz to 3 MHz to 1) differentiate the pathological stages of cancer cells under laboratory conditions and 2) permit the extraction of electrical parameters related to cellular information for further analysis. This provides evidence to form the basis of bioimpedance measurement at the cellular level and aids the potential future development of rapid diagnostics from biopsy materials. Three cell lines, representing normal breast epithelia and different pathological stages of breast cancer, have been measured using a standard impedance analyzer driving a four-electrode chamber filled with different cell suspensions. We identify the specific BIS profile for each cell type and determine whether these can be differentiated. In addition, the electrical parameters, e.g., the intracellular conductivity, membrane capacitance/capacity, characteristic frequency, are extracted by the use of equivalent circuit models and physical models to provide details of the cell electric signatures for further analysis of cancer cell pathology.

Hepatic entropy and uniformity: additional parameters that can potentially increase the effectiveness of contrast enhancement during abdominal CT.

AIM: To determine how hepatic entropy and uniformity of computed tomography (CT) images of the liver change after the administration of contrast material and to assess whether these additional parameters are more sensitive to tumour-related changes in the liver than measurements of hepatic attenuation or perfusion. MATERIALS AND METHODS: Hepatic attenuation, entropy, uniformity, and perfusion were measured using multi-phase CT following resection of colorectal cancer. Based on conventional CT and fluorodeoxyglucose positron emission tomography, 12 patients were classified as having no evidence of malignancy, eight with extra-hepatic tumours only, and eight with metastatic liver disease. RESULTS: Hepatic attenuation and entropy increased after CM administration whereas uniformity decreased. Unlike hepatic attenuation, entropy and uniformity changed maximally in the arterial phase. No significant differences in hepatic perfusion or attenuation were found between patient groups, whereas arterial-phase entropy was lower (p=0.034) and arterial-phase uniformity was higher (p=0.034) in apparently disease-free areas of liver in patients with hepatic metastases compared with those with no metastases. CONCLUSION: Temporal changes in hepatic entropy and uniformity differ from those for hepatic attenuation. By reflecting the distribution of hepatic enhancement, these additional parameters are more sensitive to tumour-related changes in the liver than measurements of hepatic attenuation or perfusion.

Electronic properties of the interface between p-CuI and anatase-phase n-TiO2 single crystal and nanoparticulate surfaces: a photoemission study.

We present a study of the growth of the p-type inorganic semiconductor CuI on n-type TiO2 anatase single crystal (101) surfaces and on nanoparticulate anatase surfaces using synchrotron radiation photoemission spectroscopy. Core level photoemission data obtained using synchrotron radiation reveal that both the substrate (TiO2) and the overlayer (CuI) core levels shift to a lower binding energy to different degrees following the growth of CuI on TiO2. Valence band photoemission data show that the valence band maximum of the clean substrate differs from that of the dosed surface which may be interpreted qualitatively as due to the introduction of a new density of states within the band gap of TiO2 as a result of the growth of CuI. The valence band offset for the heterojunction n-TiO2p-CuI has been measured using photoemission for both nanoparticulate and single crystal TiO2 surfaces, and the band energy alignment for these heterojunction interfaces is presented. With the information obtained here, it is suggested that the interface between p-CuI and single crystal anatase-phase n-TiO2 is a type-II heterojunction interface, with significant band bending. The measured total band bending matches the work function change at the interface, i.e., there is no interface dipole. In the case of the nanoparticulate interface, an interface dipole is found, but band bending within the anatase nanoparticles remains quite significant. We show that the corresponding depletion layer may be accommodated within the dimension of the nanoparticles. The results are discussed in the context of the functional properties of dye-sensitized solid state solar cells.

Interaction of multiple distortions in spatial light modulators.

Spatial light modulators are the key components in real-time optical image-processing systems. The phase and the intensity of their outputs will often depart from ideal behavior. An experimental method is described that permits the effects of multiple distortions, present simultaneously, to be modeled. A computer simulation of a bismuth silicon oxide-based correlator is presented, with spatial light modulators subject to three types of distortion, including phase and amplitude. The experimental method permits both the main effects of the distortions and their interactions to be predicted. Combining all the distortions simultaneously gives a more accurate assessment of the suitability of a spatial light modulator for a given optical processing task. Images of 256 × 256 pixels were used, and the simulation took 15 min. with a Sun SPARCstation 2.

Multilevel phase- and amplitude-encoded modified-filter synthetic-discriminant-function filters.

The performance of the modified-filter synthetic-discriminant-function (MfSDF) filter with multilevel phase and amplitude (MLAP) constraints is investigated with various in-plane rotated images from an in-class Bradley armored personnel carrier vehicle and an out-of-class Abram MI tank; this is of interest because of the commercial availability of liquid-crystal televisions, which are able to encode the gray-level amplitude and/or the discrete multilevel phase information. The evaluation is performed to explain better the image-distortion range that can be covered effectively by MLAP/MfSDF filters. The results show that the MLAP/MfSDF filter offers much-improved correlator system performance with a greater allowable image-distortion range while maintaining 100% discrimination between in-class and out-ofclass images; furthermore, it shows an improved ability to accommodate the input image noise when compared with the MfSDF filter with a binary phase-only constraint. Thus the MLAP/MfSDF can be implemented effectively by a hybrid optical/digital correlator system to track a vehicle or a tank dynamically as it moves along a random trajectory across the input field.

Modified filter synthetic discriminant functions for improved optical correlator performance.

By the filter modulation operator N, the modified filter synthetic discriminant function permits advantageous preprocessing of individual training-set images that are used in a linear combination to construct the filter synthetic discriminant function, which applies a modulation operator M to the synthetic discriminant function. A relaxation algorithm is used to satisfy the equal correlation peaks rule in the correlator output plane. As the filter modulation operators M and N can be given any functional form, the modified filter synthetic discriminant function design proposed is sufficiently general to be described as a unified filter modulation synthetic discriminant function design.

Two-pixel computer-generated hologram with a zero-twist nematic liquid-crystal spatial light modulator.

We present a method of producing a computer-generated hologram by use of a zero-twist linear nematic liquid-crystal spatial light modulator. A 2x1 macro pixel method is used; one pixel represents the real data, and one, the imaginary. A method is shown that produces both positive and negative analog amplitude modulation.

Dynamic complex wave-front modulation with an analog spatial light modulator.

A method of producing an arbitrary complex field modulation by use of two pixels of an analog ferroelectric spatial light modulator (SLM) is demonstrated. The method uses the gray-scale modulation capabilities of a SLM to spatially encode the complex data on two pixels. A spatial filter is used to remove the carrier signal. This technique gives fast gray-level amplitude and phase modulation.

Prophylactic intravenous mannitol and normal saline in patients with poor renal function prior to cardiac surgery: time for a multicentre trial?

BACKGROUND: Postoperative acute renal failure requiring dialysis has a poor prognosis, which has remained unaltered for 50 years. Therefore, in cardiac surgical patients at increased risk of postoperative oliguric acute renal failure (preoperative serum creatinine >0.13 mmol/L), we assessed the use of prophylactic intravenous (i.v) 20% mannitol and normal saline therapy in addition to traditional methods of therapeutic renal support. METHODS: Seventy-five patients with a mean preoperative serum creatinine of 0.192 mmol/L received i.v. 20% mannitol and normal saline pre-, intra- and postoperatively. This treatment was continued postoperatively until serum creatinine returned to baseline. RESULTS: No patient required dialysis, no patient died, developed a myocardial infarction nor a stroke in the first 30 days post-surgery. Serum creatinine and urea increased to a mean peak on day 3 of 0.233 +/- 94 and 24.6 +/- 13 mmol/L, respectively. However, all patients maintained a high urine output (>2 L/day) and no patient required haemodialysis. CONCLUSION: Therapy with i.v. 20% mannitol and normal saline appears safe and effective in maintaining a diuresis and may avoid the need for dialysis. A randomised controlled trial of this treatment in patients at increased risk of postoperative acute renal failure is warranted.

Modified fringe-adjusted joint transform correlation to accommodate noise in the input scene.

A modified fringe-adjusted joint transform correlator is proposed that is able to accommodate noise in the input scene. The effect of noise in the input scene on the performance of the joint transform correlator is analyzed and quantified. When the target is embeddedin aseverely noise-corrupted input scene, it is shown that the proposed modified fringe-adjusted filter joint transform correlator delivers a better correlation performance and the capacity to accommodate this noise than does the fringe-adjusted filter-based correlator. When the power spectra of the input image and the reference image are subtracted from the power spectrum of the joint-input image, it is found that the noise effect on the output plane is independent of the objects in the input scene and originates from the convolution of the reference image and noise in the input scene.

High-speed, acousto-optically addressed optical memory.

A page-oriented, angle-multiplexed volume holographic optical-memory recording system has been constructed. This memory is addressed by the use of an acousto-optic deflector with a random-access time of 16 µs per page. This enables data transfer rates of 5.28 Gbits/s when pages of binary data are being stored. The reconstruction quality of images stored as memory pages is assessed with the quality achieved with the acousto-optic device compared with that achieved with the original recording optics.

Application of nonlinearity to wavelet-transformed images to improve correlation filter performance.

A useful filter for pattern recognition must strike a compromise between the conflicting requirements of in-class distortion tolerance and out-of-class discrimination. Such a filter will be bandpass in nature, the high-frequency response being attenuated to provide less sensitivity to in-class variations, while the low frequencies must be removed, since they compromise the discrimination ability of the filter. A convenient bandpass is the difference of Gaussian (DOG) function, which provides a close approximation to the Laplacian of Gaussian. We describe the effect of a preprocessing operation applied to a DOG filtered image. This operation is shown to result in greater tolerance to in-class variation while maintaining an excellent discrimination ability. Additionally, the introduction of nonlinearity is shown to provide greater robustness in the filter response to noise and background clutter in the input scene.

Synthetic discriminant function filter employing nonlinear space-domain preprocessing on bandpass-filtered images.

Previously [Appl. Opt. 36, p. 9212 (1997)] we examined the performance of the linear and nonlinear preprocessed difference-of-Gaussians filter, and it was shown that this operation results in greater tolerance to in-class variations while maintaining excellent discrimination ability. The introduction of nonlinearity was shown to provide greater robustness to the filter's response to noise and background clutter in the input scene. We incorporate this new operation into the synthesis of a modified synthetic discriminant function filter. The filter is shown to produce sharp peaks, excellent discrimination without the need to include out-of-class objects, and good invariance to out-of-plane rotation over a distortion range of up to 90 degrees . Additionally, the introduction of nonlinearity is shown to provide greater robustness of the filter response to background clutter in the input scene.

UV Microstereolithography System that uses Spatial Light Modulator Technology.

A new stereophotolithography technique utilizing a spatial light modulator (SLM) to create three-dimensional components with a planar, layer-by-layer process of exposure is described. With this procedure it is possible to build components with dimensions in the range of 50 mum-50 mm and feature sizes as small as 5 mum with a resolution of 1 mum. A polysilicon thin-film twisted nematic SVGA SLM is used as the dynamic photolithographic mask. The system consists of eight elements: a UV laser light source, an optical shutter, beam-conditioning optics, a SLM, a multielement reduction lens system, a high-resolution translation stage, a control system, and a computer-aided-design system. Each of these system components is briefly described. In addition, the optical characteristics of commercially available UV curable resins are investigated with nondegenerate four-wave mixing. Holographic gratings were written at a wavelength of 351.1 nm and read at 632.8 nm to compare the reactivity, curing speed, shrinkage, and resolution of the resins. These experiments were carried out to prove the suitability of these photopolymerization systems for microstereolithography.

Experimental implementation of a Wiener filter in a hybrid digital--optical correlator.

We present the implementation of a clutter-tolerant filter in a hybrid correlator system. Wiener filters were mapped with a complex encoding technique onto a smectic A(*) liquid-crystal spatial light modulator (SLM). The technique overcomes the problem of representing high-dynamic-range data on SLM's that have limited modulation capabilities. It also provides a compact image recognition system that is robust enough for many real-world applications. Experimental results are presented.