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Modulating the band gap of two-dimensional (2D) transition metal dichalcogenide (TMDC) semiconductors is critical for their application in a wider spectral range. Alloying has been demonstrated as an effective method for regulating the band gap of 2D TMDC semiconductors. The fabrication of large-area 2D TMDC alloy films with centimeter-scale uniformity is fundamental to the application of integrated devices. Herein, we report a liquid-phase precursor one-step chemical vapor deposition (CVD) method for fabricating a MoxW1-xS2 alloy monolayer with a large size and an adjustable band gap. Good crystalline quality and high uniformity on a wafer scale enable the continuous adjustment of its band gap in the range of 1.8-2.0 eV. Density functional theory calculations provided a deep understanding of the Raman-active vibration modes of the MoxW1-xS2 alloy monolayer and the change in the conductivity of the alloy with photon energy. The synthesis of large-area MoxW1-xS2 alloy monolayers is a critical step toward the application of 2D layered semiconductors in practical optoelectronic devices.
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The development of efficient electrocatalysts for the hydrogen evolution reaction (HER) holds immense importance in the context of large-scale hydrogen production from water. Nevertheless, the practical application of such catalysts still relies on precious platinum-based materials. There is a pressing need to design high-performing, non-precious metal electrocatalysts capable of generating hydrogen at substantial current levels. We report here a stable monolith catalyst of Te-doped-WSe2 directly supported by a highly conductive W mesh. This catalyst demonstrates outstanding electrocatalytic performance and stability in acidic electrolytes, especially under high current conditions, surpassing the capabilities of commercial 5% Pt/C catalysts. Specifically, at current densities of 10 and 1200 mA cm-2, it exhibits a minimal overpotential of 79 and 232 mV, along with a small Tafel slope of 55 mV dec-1, respectively. The remarkable catalytic activity of Te-WSe2 can be attributed to the exceptional electron transfer facilitated by the stable monolithic structure, as well as the abundant and efficient active sites in the material. In addition, density functional theory calculations further indicate that Te doping adjusts H atom adsorption on various positions of WSe2, making it closer to thermal neutrality compared to the original material. This study presents an innovative approach to develop cost-effective HER electrocatalysts that perform optimally under high current density conditions.
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BACKGROUND: BTRX-246040, a nociceptin/orphanin FQ peptide receptor antagonist, is being developed for the treatment of depressive patients. However, the underlying mechanism of this potential antidepressant is still largely unclear. Here, we studied the antidepressant-related actions of BTRX-246040 in the ventrolateral periaqueductal gray (vlPAG). METHODS: The tail suspension test, forced swim test, female urine sniffing test, sucrose preference test, and learned helplessness (LH) combined with pharmacological approaches were employed to examine the antidepressant-like effects and drug effects on LH-induced depressive-like behavior in C57BL/6J mice. Electrophysiological recordings in vlPAG neurons were used to study synaptic activity. RESULTS: Intraperitoneal administration of BTRX-246040 produced antidepressant-like behavioral effects in a dose-dependent manner. Systemic BTRX-246040 (10 mg/kg) resulted in an increased frequency and amplitude of miniature excitatory postsynaptic currents (EPSCs) in the vlPAG. Moreover, slice perfusion of BTRX-246040 directly elevated the frequency and amplitude of miniature EPSCs and enhanced the evoked EPSCs in the vlPAG, which were blocked by pretreatment with the nociceptin/orphanin FQ peptide receptor agonist Ro 64-6198. In addition, intra-vlPAG application of BTRX-246040 produced antidepressant-like behavioral effects in a dose-dependent manner. Moreover, intra-vlPAG pretreatment with 6-cyano-7-nitroquinoxaline-2,3-dione reversed both systemic and local BTRX-246040-mediated antidepressant-like behavioral effects. Furthermore, both systemic and local BTRX-246040 decreased the LH phenotype and reduced LH-induced depressive-like behavior. CONCLUSIONS: The results suggested that BTRX-246040 may act through the vlPAG to exert antidepressant-relevant actions. The present study provides new insight into a vlPAG-dependent mechanism underlying the antidepressant-like actions of BTRX-246040.
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Neuronas , Sustancia Gris Periacueductal , Ratones , Femenino , Animales , Ratones Endogámicos C57BL , Antidepresivos/farmacología , Receptores de PéptidosRESUMEN
Glaucoma is a chronic neurodegenerative disease characterized by the progressive loss of retinal ganglion cells (RGCs). Mitochondrial DNA (mtDNA) alterations have been documented as a key component of many neurodegenerative disorders. However, whether mtDNA alterations contribute to the progressive loss of RGCs and the mechanism whereby this phenomenon could occur are poorly understood. We investigated mtDNA alterations in RGCs using a rat model of chronic intraocular hypertension and explored the mechanisms underlying progressive RGC loss. We demonstrate that the mtDNA damage and mutations triggered by intraocular pressure (IOP) elevation are initiating, crucial events in a cascade leading to progressive RGC loss. Damage to and mutation of mtDNA, mitochondrial dysfunction, reduced levels of mtDNA repair/replication enzymes, and elevated reactive oxygen species form a positive feedback loop that produces irreversible mtDNA damage and mutation and contributes to progressive RGC loss, which occurs even after a return to normal IOP. Furthermore, we demonstrate that mtDNA damage and mutations increase the vulnerability of RGCs to elevated IOP and glutamate levels, which are among the most common glaucoma insults. This study suggests that therapeutic approaches that target mtDNA maintenance and repair and that promote energy production may prevent the progressive death of RGCs.
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Daño del ADN , ADN Mitocondrial , Glaucoma/genética , Glaucoma/fisiopatología , Mutación , Células Ganglionares de la Retina/fisiología , Animales , Apoptosis/fisiología , Axones/patología , Axones/fisiología , Supervivencia Celular/genética , Supervivencia Celular/fisiología , Reparación del ADN , Modelos Animales de Enfermedad , Progresión de la Enfermedad , Glaucoma/patología , Ácido Glutámico/metabolismo , Presión Intraocular/genética , Presión Intraocular/fisiología , Masculino , Mitocondrias/genética , Mitocondrias/patología , Mitocondrias/fisiología , Nervio Óptico/patología , Nervio Óptico/fisiopatología , Ratas Wistar , Células Ganglionares de la Retina/patología , Factores de TiempoRESUMEN
OBJECTIVE: To investigate the dynamic changes and molecular mechanisms of thioredoxin system in lateral geniculate body of chronic intraocular hypertension rats. METHOD: Glaucoma model was established by cauterizing rat's episclera veins. Right eye is the treatment group, while left eye is the sham operation group, vein of which is just isolated not be cauterized. There were 25 rats in high intraocular pressure group, and 4 rats in control group. The expression of Trx, Trx1, Trx2, Sesn2, Srxn1, Txnip was detected by Western blot and RT-PCR at different time points after intraocular pressure rose (1, 3, 7, 14, 28 d), and were grouped according to the time points. Paired t test was used in the data analysis within each time group and one-way ANOVA was used in the comparisons among different time groups. RESULT: After 3 days of glaucoma induction, the gray-scale value of thioredoxin (0.83 ± 0.02) was statistically significant decreased (F = 4.871, P = 0.005), compared with the control group (1.02 ± 0.04). After 14 days, TRX1 related expression level reduced to 0.63 ± 0.04, while the control group level was 0.96 ± 0.03 (t = 13.647, P < 0.05). The related expression level of SESN2 was 0.53 ± 0.11, but the control group expression was 0.96 ± 0.03 (t = 13.812, P < 0.05). SRXN1 decreased to 0.71 ± 0.05 and 0.49 ± 0.03 after 7 days and 14 days respectively, while the expression of control group were 0.97 ± 0.02 and 0.96 ± 0.02 respectively. (Group 7 d, t = 9.451, P < 0.05; group 14 d, t = 13.611, P < 0.01).On the contrary, expression of TXINP was upregulated to 1.83 ± 0.04 and 2.37 ± 0.03 respectively, while the expression of control group were 1.00 ± 0.02 and, 0.95 ± 0.03 (Group 7 d, t = 7.924, P < 0.05;14 d t = 12.637, P < 0.01) respectively. CONCLUSIONS: Oxidative stress existed in lateral geniculate body of chronic intraocular hypertension rat model, and the down-regulated expression of Trx, Trx2 of the Trx system in lateral geniculate body was inhibited by multiple pathways when oxidative damage occurs. The inhibitor genes on up-stream increased while promoting genes diminished.
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Cuerpos Geniculados/metabolismo , Glaucoma/metabolismo , Tiorredoxinas/fisiología , Animales , Regulación hacia Abajo , Presión Intraocular , Hipertensión Ocular/metabolismo , Oxidación-Reducción , Estrés Oxidativo , Ratas , Factores de Tiempo , Regulación hacia ArribaRESUMEN
Skin cancer is one of the most common malignant tumors worldwide, and early detection is crucial for improving its cure rate. In the field of medical imaging, accurate segmentation of lesion areas within skin images is essential for precise diagnosis and effective treatment. Due to the capacity of deep learning models to conduct adaptive feature learning through end-to-end training, they have been widely applied in medical image segmentation tasks. However, challenges such as boundary ambiguity between normal skin and lesion areas, significant variations in the size and shape of lesion areas, and different types of lesions in different samples pose significant obstacles to skin lesion segmentation. Therefore, this study introduces a novel network model called HDS-Net (Hybrid Dynamic Sparse Network), aiming to address the challenges of boundary ambiguity and variations in lesion areas in skin image segmentation. Specifically, the proposed hybrid encoder can effectively extract local feature information and integrate it with global features. Additionally, a dynamic sparse attention mechanism is introduced, mitigating the impact of irrelevant redundancies on segmentation performance by precisely controlling the sparsity ratio. Experimental results on multiple public datasets demonstrate a significant improvement in Dice coefficients, reaching 0.914, 0.857, and 0.898, respectively.
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Enfermedades de la Piel , Neoplasias Cutáneas , Humanos , Enfermedades de la Piel/diagnóstico por imagen , Piel/diagnóstico por imagen , Neoplasias Cutáneas/diagnóstico por imagen , Procesamiento de Imagen Asistido por ComputadorRESUMEN
In this study, a novel heterogeneous double-wire arc additive manufacturing method was used for the in-situ synthesis of a novel Ti-Ni-Al-V alloy wall. The results indicated that the synthesis wall was composed of the NiTi2, B2, and Ni4Ti3 phases from the bottom to the top. With an increase in deposition layers, the Ti2Ni content decreased. The hardness at the bottom was â¼685.4 HV0.2, while that of the middle and stable regions was 553 HV0.2. The maximum compressive strength was 2100 MPa. The fracture morphology was brittle. After cyclic compression, the recoverable and unrecoverable strains were 4.79 % and 1.21 %, respectively, indicating excellent recovery characteristics.
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OBJECTIVE: To conduct a systematic review with meta-analyses to assess the recent scientific literature addressing the application of deep learning radiomics in oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS: Electronic and manual literature retrieval was performed using PubMed, Web of Science, EMbase, Ovid-MEDLINE, and IEEE databases from 2012 to 2023. The ROBINS-I tool was used for quality evaluation; random-effects model was used; and results were reported according to the PRISMA statement. RESULTS: A total of 26 studies involving 64,731 medical images were included in quantitative synthesis. The meta-analysis showed that, the pooled sensitivity and specificity were 0.88 (95 %CI: 0.87â¼0.88) and 0.80 (95 %CI: 0.80â¼0.81), respectively. Deeks' asymmetry test revealed there existed slight publication bias (P = 0.03). CONCLUSIONS: The advances in the application of radiomics combined with learning algorithm in OSCC were reviewed, including diagnosis and differential diagnosis of OSCC, efficacy assessment and prognosis prediction. The demerits of deep learning radiomics at the current stage and its future development direction aimed at medical imaging diagnosis were also summarized and analyzed at the end of the article.
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Carcinoma de Células Escamosas , Aprendizaje Profundo , Neoplasias de la Boca , Humanos , Neoplasias de la Boca/diagnóstico por imagen , Neoplasias de la Boca/diagnóstico , Neoplasias de la Boca/patología , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/diagnóstico por imagen , Carcinoma de Células Escamosas/patología , Sensibilidad y Especificidad , RadiómicaRESUMEN
Wire arc additive manufacturing (WAAM) excels in producing medium to large components with a high deposition rate. Process optimization is crucial for uniform, defect-free components. This research employs orthogonal experimental design and response surface methodology (RSM) to control TIG WAAM-ed 308L stainless steel components. Varied parameters, including tungsten electrode angle, welding current, and speed, target weld bead attributes. Analysis of variance (ANOVA) evaluates multi-processing parameter influence on weld bead formation. Comparison with experimental results confirms accurate modeling of the relationship between parameters and bead attributes. The study optimizes process parameters and swing to enhance dimensional accuracy in single-layer and multi-layer components, improving precision, quality, and accuracy in thin-walled structures.
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Light is an underappreciated mood manipulator. People are often exposed to electronic equipment, which results in nocturnal blue light exposure in modern society. Light pollution drastically shortens the night phase of the circadian rhythm. Preclinical and clinical studies have reported that nocturnal light exposure can influence mood, such as depressive-like phenotypes. However, the effects of blue light at night (BLAN) on other moods and how it alters mood remain unclear. Here, we explored the impact of BLAN on stress-provoked aggression in male SpragueâDawley rats, focusing on its influence on basolateral amygdala (BLA) activity. Resident-intruder tests, extracellular electrophysiological recordings, and enzyme-linked immunosorbent assays were performed. The results indicated that BLAN produces stress-induced heightened aggressive and anxiety-like phenotypes. Moreover, BLAN not only potentiates long-term potentiation and long-term depression in the BLA but also results in stress-induced elevation of brain-derived neurotrophic factor (BDNF), mature BDNF, and phosphorylation of tyrosine receptor kinase B expression in the BLA. Intra-BLA microinfusion of BDNF RNAi, BDNF neutralizing antibody, K252a, and rapamycin blocked stress-induced heightened aggressive behavior in BLAN rats. In addition, intra-BLA application of BDNF and 7,8-DHF caused stress-induced heightened aggressive behavior in naïve rats. Collectively, these results suggest that BLAN results in stress-evoked heightened aggressive phenotypes, which may work by enhancing BLA BDNF signaling and synaptic plasticity. This study reveals that nocturnal blue light exposure may have an impact on stress-provoked aggression. Moreover, this study provides novel insights into the BLA BDNF-dependent mechanism underlying the impact of the BLAN on mood.
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Brain-selective kinase 2 (BRSK2) has been shown to play an essential role in neuronal polarization. In the present study, we show that BRSK2 is also abundantly expressed in pancreatic islets and MIN6 ß-cell line. Yeast two-hybrid screening, GST fusion protein pull-down, and co-immunoprecipitation assays reveal that BRSK2 interacts with CDK-related protein kinase PCTAIRE1, a kinase involved in neurite outgrowth and neurotransmitter release. In MIN6 cells, BRSK2 co-localizes with PCTAIRE1 in the cytoplasm and phosphorylates one of its serine residues, Ser-12. Phosphorylation of PCTAIRE1 by BRSK2 reduces glucose-stimulated insulin secretion (GSIS) in MIN6 cells. Conversely, knockdown of BRSK2 by siRNA increases serum insulin levels in mice. Our results reveal a novel function of BRSK2 in the regulation of GSIS in ß-cells via a PCTAIRE1-dependent mechanism and suggest that BRSK2 is an attractive target for developing novel diabetic drugs.
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Quinasas Ciclina-Dependientes/metabolismo , Glucosa/metabolismo , Células Secretoras de Insulina/metabolismo , Insulina/metabolismo , Proteínas Serina-Treonina Quinasas/metabolismo , Animales , Línea Celular , Quinasas Ciclina-Dependientes/genética , Citoplasma/genética , Citoplasma/metabolismo , Técnicas de Silenciamiento del Gen , Glucosa/genética , Humanos , Insulina/genética , Secreción de Insulina , Células Secretoras de Insulina/citología , Masculino , Ratones , Ratones Endogámicos BALB C , Fosforilación/fisiología , Proteínas Serina-Treonina Quinasas/genéticaRESUMEN
In this paper, a porous polyurethane sponge with excellent hydrophobicity was prepared through thermal phase separation. Preparation condition modified experiments were systematically carried out, and a sponge with a saturated oil absorption capacity (13.3 g g-1) and a rapid absorption rate (achieving absorption equilibrium within 20 s) was achieved. The thermoplastic polyurethane (TPU) sponge as an oil absorbent is capable of selectively absorbing various oils/organic solvents from oil/water mixtures with a high recovery rate. To further enhance the hydrophobicity and mechanical properties of the porous sponge, 3% reduced graphene oxide was doped to this material. The morphological investigation indicated that the three-dimensional composite sponges have uniformly distributed micropores and nanopores, and the hydrophobicity and mechanical properties were improved. The composite as a whole exhibited remarkable superelasticity, excellent reversible compressibility, and fatigue resistance (strength up to 186 kPa at 80% strain), which allows it to re-absorb oil by simple manual extrusion. The abovementioned properties make this TPU porous material a promising candidate for practical application in water pollution treatment.
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A novel Chinese-style sausage with Chinese traditional fermented condiments used as additional ingredients is produced in this study. The aim of this study was to investigate the microbial community's structure, the volatile flavor substances and their potential correlation in the novel Chinese sausage. High-throughput sequencing (HTS) and solid-phase microextraction-gas chromatography-mass spectrometry (GC-MS) were, respectively, used to analyze the microbial diversity and volatile flavor substances of the novel Chinese-style sausage during storage. The results showed that Firmicutes, Proteobacteria and Actinobacteria were the predominant bacterial genera, and Hyphopichia and Candida were the predominant fungal genera. A total of 88 volatile flavor substances were identified through GC-MS, among which 18 differential flavor compounds were screened (VIP > 1), which could be used as potential biomarkers to distinguish the novel sausages stored for different periods. Lactobacillus exhibited a significant negative correlation with 2,3-epoxy-4,4-dimethylpentane and acetoin and a significant positive correlation with 2-phenyl-2-butenal. Hyphopichia significantly positively correlated with ester. Leuconostoc significantly positively correlated with ethyl caprate, ethyl palmate, ethyl tetradecanoate and ethyl oleate while it negatively correlated with hexanal. This study provides a theoretical basis for revealing the flavor formation mechanisms and the screening of functional strains for improving the flavor quality of the novel Chinese-style sausage.
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Electrocatalytic hydrogen evolution reaction (HER) is receiving increasing attention as an effective process to produce clean energy. The commonly used precious metal catalysts can be hybridized with semiconductors to form heterostructures for the improvement of catalytic efficiency and reduction of cost. It will be promising to further improve the efficiency of heterostructure-based nanocatalysts in electrocatalytic and photocatalytic HER using a simple and effective method. Herein, we improve the efficiency of Au/TiO2 in electrocatalytic and photo-electrocatalytic HER by selectively adsorbing p-aminothiophenol (PATP) molecules. The PATP molecules are adsorbed on the gold surface by using a simple solution-based method and favor the charge separation at the Au-TiO2 interface. We also compare the PATP molecules with other thiophenol molecules in the enhancement of electrocatalytic HER. The PATP-induced enhancement in electrocatalysis is then further investigated by density functional theory (DFT) calculations, and this enhancement is attributed to a reduction in Gibbs energy of adsorbed hydrogen after surface adsorption of PATP molecules. This work provides a simple, cost-effective, and highly efficient approach to improve the electrocatalytic and photo-electrocatalytic efficiency of Au/TiO2, and this approach could be easily extended to other heterostructure-based nanocatalysts for performance enhancement and may be used in many other catalytic reactions.
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Plasmon-mediated electrochemistry is an emerging area of interest in which the electrochemical reactions are enhanced by employing metal nanostructures possessing localized surface plasmon resonance (LSPR). However, the reaction efficacy is still far below its theoretical limit due to the ultrafast relaxation of LSPR-generated hot carriers. Herein, we introduce p-hydroxythiophenol (PHTP) as a molecular cocatalyst to significantly improve the reaction efficacy in plasmon-mediated electrochemical oxidation of p-aminothiophenol (PATP) on gold nanoparticles. Using electrochemical techniques, in situ Raman spectroscopy, and theoretical calculations, we elucidate that the presence of PHTP improves the hot hole-mediated electrochemical oxidation of PATP by 2-fold through the trapping of plasmon-mediated hot electrons. In addition, the selectivity of PATP oxidation could also be modulated by the introduction of PHTP cocatalyst. This tactic of employing molecular cocatalyst can be drawn out to endorse various plasmonic electrochemical reactions because of its simple protocol, high efficiency, and high selectivity.
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Yatakemycin (YTM), an antitumor natural product, represents the most potent member of a class of potent anticancer natural products including CC-1065 and duocarmycins. Herein we describe the biosynthetic gene cluster of YTM, which was identified by genome scanning of Streptomyces sp. TP-A0356. This cluster consists of 31 open reading frames (ORFs) and was localized to a 36 kb DNA segment. Moreover, its involvement in YTM biosynthesis was confirmed by cluster deletion, gene replacement, and complementation. Inactivation of ytkT, which encodes a radical S-adenosylmethionine (SAM) protein, created a mutant strain that failed to produce YTM but accumulated a new metabolite, which was structurally elucidated as a precursor that was related to the formation of the cyclopropane ring. More importantly, biochemical characterization of the radical SAM-dependent enzyme YtkT revealed that it is a novel C-methyltransferase and contributes to an advanced intermediate during formation of the cyclopropane ring through a radical mechanism in the YTM biosynthetic pathway. On the basis of in silico analysis, genetic experiments, structure elucidation of the novel intermediate, and biochemical characterization, a biosynthetic pathway for yatakemycin was proposed, which sets the stage to further investigate the novel enzymatic mechanisms and engineer the biosynthetic machinery for the production of novel analogues.
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Antineoplásicos/metabolismo , Ciclopropanos/química , Indoles/metabolismo , Metiltransferasas/metabolismo , Familia de Multigenes , Pirroles/metabolismo , S-Adenosilmetionina/metabolismo , Compuestos de Espiro/metabolismo , Productos Biológicos/metabolismo , Duocarmicinas , Metiltransferasas/genética , Reproducibilidad de los Resultados , Compuestos de Espiro/química , Streptomyces/enzimología , Streptomyces/genética , Streptomyces/metabolismoRESUMEN
Debate has been warranted as a meaningful activity to promote students' higher-level thinking, such as critical thinking. However, traditional debate activities which are typically carried out in the physical classroom may meet some obstructions of limited time and space, which would result in the phenomenon that many participants act as silent watchers rather than mind exchangers. Moreover, it is hard to make a visualized record about the whole process and contents of the traditional debate activity. The current study aimed to explore the effects of argument map (AM)-supported online group debate activities on college students' critical thinking, including their depth and phases of critical thinking, and the relationship between students' depth of critical thinking and their number of speeches. In the study, an innovative argumentation construction way was designed. All students enrolled in a course could have a chance to attend the AM-supported online group debate activities and the whole process and outcomes of online group debate could be visualized by AM. At the same time, the effectiveness of the innovative activities was evaluated by content analysis of AM. A total of 42 sophomores in the undergraduate course named "Learning Sciences and Technology" were recruited to attend online group debate activities assisted by a web-based visualization tool named "ZJU Yuque" in 5 weeks. Newman's framework about the depth of critical thinking and Murthy's instrument of critical thinking phases were employed as guidelines. We found that 42 students' overall depth of critical thinking was gradually improved in an obvious way during the five online debate activities. The most frequent phases for students in the first and second team in online group debate activities include UnderstandâUnderstand (UâU), RecognizeâUnderstand (RâU), and UnderstandâEvaluate (UâE). However, students' creating behaviors were not significantly generated. Teachers' real-time feedback was helpful for students' improvements of high-level thinking skills and their preparation for the next debate activity. Students' interviews found that students highly valued such online group debate activities because every student could have a chance to express their thoughts and they had enough time to prepare debate contents. Based on the findings, some implications were proposed for the better design and implementation of online group debate activities.
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The dynein protein plays a key role in the degradation pathway by attaching to targeted molecules and transporting the autophagosome to the centrosome. Aging plays an important role in the pathogenesis of Parkinson's disease (PD), but its effect on dynein is not clear. In this study we analyzed behavioral characteristics using the rod endurance test and climbing rod time test in different aged mice (3 months, 12 months, 20 months), and measured protein expression of dynein, α-synuclein, Tctex-1, and LC3 in the substantianigra of the mice by Western blot. The mRNA levels of dynein, α-synuclein, LC3 and Tctex-1 were measured by quantitative real time reverse transcription PCR, and detecting expression of dynein and α-synuclein by immunofluorescence. We found the motor functions of A53T mutant mice, in 12 months and 20 months, decreased more significantly compared with normal mice (p < 0.05). In addition, the expression of dynein, LC3-â ¡ and Tctex-1 proteins in the substantia nigra of the two groups decreased with age. However, α-synuclein protein increased gradually with age, with significantly higher levels in the PD groups compared with age matched controls (p < 0.05). These results were confirmed by immunofluorescence. Our data demonstrates that dynein and other autophagy proteins change with age, and this is associated with increased α-synuclein. Therefore, therapeutics that prevent dynein dysfunction may offer novel treatments for PD and other autophagy related diseases.
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Enfermedad de Parkinson , alfa-Sinucleína , Envejecimiento , Animales , Dineínas/metabolismo , Ratones , Ratones Transgénicos , Enfermedad de Parkinson/genética , Sustancia Negra , alfa-Sinucleína/genética , alfa-Sinucleína/metabolismoRESUMEN
It is well known that oil stranded on shoreline substrates can be difficult to remove and cause serious environmental effects. To address this issue, a calcium alginate-cellulose nanocrystal (CA-CNC)-based coating with a unique surface structure and superhydrophobic properties was developed to reduce the extent of shoreline oiling. The results of batch washing test showed that not only did the introduction of CNC not reduce the oil removal efficiency; it also improved the environmental stability of the coating to resist the effects associated with seawater immersion and erosion (especially in the case of 0.4 wt% of CNC). The oil-repellent performance of the coated gravels implied that both oscillation time and oil concentration had almost no effects on the amount of adhered oil. Assessment of oiling prevention based on the laboratory shoreline tank simulator proved the coated gravel performed very well as more oil floated and less oil remained on substrates and penetrated into the subsurface. Biotoxicity analysis showed that the coating powders reduced impacts on the toxicity of the oil to algae at low doses. There is a good potential for the use of this CA-CNC based coating technique to improve shoreline oil spill response.
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Nanopartículas , Contaminación por Petróleo , Contaminantes Químicos del Agua , Alginatos , Celulosa , Contaminación por Petróleo/análisis , Contaminantes Químicos del Agua/químicaRESUMEN
All-inorganic perovskite quantum dots (QDs) CsPbX3 (X = Cl, Br, and I) have recently emerged as a new promising class of X-ray scintillators. However, the instability of perovskite QDs and the strong optical scattering of the thick opaque QD scintillator film imped it to realize high-quality and robust X-ray image. Herein, the europium (Eu) doped CsPbBr3 QDs are in situ grown inside transparent amorphous matrix to form glass-ceramic (GC) scintillator with glass phase serving as both matrix and encapsulation for the perovskite QD scintillators. The small amount of Eu dopant optimizes the crystallization of CsPbBr3 QDs and makes their distribution more uniform in the glass matrix, which can significantly reduce the light scattering and also enhance the photoluminescence emission of CsPbBr3 QDs. As a result, a remarkably high spatial resolution of 15.0 lp mm-1 is realized thanks to the reduced light scattering, which is so far a record resolution for perovskite scintillator based X-ray imaging, and the scintillation stability is also significantly improved compared to the bare perovskite QD scintillators. Those results provide an effective platform particularly for the emerging perovskite nanocrystal scintillators to reduce light scattering and improve radiation hardness.