Measuring initial enamel erosion with quantitative light-induced fluorescence and optical coherence tomography: an in vitro validation study.

BACKGROUND: Measurement of initial enamel erosion is currently limited to in vitro methods. Optical coherence tomography (OCT) and quantitative light-induced fluorescence (QLF) have been used clinically to study advanced erosion. Little is known about their potential on initial enamel erosion. OBJECTIVES: To evaluate the sensitivity of QLF and OCT in detecting initial dental erosion in vitro. METHODS: 12 human incisors were embedded in resin except for a window on the buccal surface. Bonding agent was applied to half of the window, creating an exposed and non-exposed area. Baseline measurements were taken with QLF, OCT and surface microhardness. Samples were immersed in orange juice for 60 min and measurements taken stepwise every 10 min. QLF was used to compare the loss of fluorescence between the two areas. The OCT system, OCS1300SS (Thorlabs Ltd.), was used to record the intensity of backscattered light of both areas. Multiple linear regression and paired t test were used to compare the change of the outcome measures. RESULTS: All 3 instruments demonstrated significant dose responses with the erosive challenge interval (p < 0.05) and a detection threshold of 10 min from baseline. Thereafter, surface microhardness demonstrated significant changes after every 10 min of erosion, QLF at 4 erosive intervals (20, 40, 50 and 60 min) while OCT at only 2 (50 and 60 min). CONCLUSION: It can be concluded that OCT and QLF were able to detect demineralization after 10 min of erosive challenge and could be used to monitor the progression of demineralization of initial enamel erosion in vitro.

Clinical studies of dental erosion and erosive wear.

We define erosion as a partial demineralisation of enamel or dentine by intrinsic or extrinsic acids and erosive tooth wear as the accelerated loss of dental hard tissue through the combined effect of erosion and mechanical wear (abrasion and attrition) on the tooth surface. Most experts believe that during the last decade there has been a significant increase in the prevalence and severity of erosive tooth wear, particularly in adolescents. Even when erosive wear occurs in its milder forms, this is a matter of concern, as it may compromise the integrity of an otherwise healthy dentition in later life. The erosive wear process is complicated and modified by many chemical, behavioural and associated processes in the mouth. If interventions are to be developed it is therefore important that in vivo methods are developed to assess the outcomes of the erosion and erosive wear processes and the effects of interventions upon them. This paper discusses potential methods of investigating erosion and erosive wear in vivo and the difficulties associated with clinical studies.

A Review of Mechano-Biochemical Models for Testing Composite Restorations.

Due to the severe mechano-biochemical conditions in the oral cavity, many dental restorations will degrade and eventually fail. For teeth restored with resin composite, the major modes of failure are secondary caries and fracture of the tooth or restoration. While clinical studies can answer some of the more practical questions, such as the rate of failure, fundamental understanding on the failure mechanism can be obtained from laboratory studies using simplified models more effectively. Reviewed in this article are the 4 main types of models used to study the degradation of resin-composite restorations, namely, animal, human in vivo or in situ, in vitro biofilm, and in vitro chemical models. The characteristics, advantages, and disadvantages of these models are discussed and compared. The tooth-restoration interface is widely considered the weakest link in a resin composite restoration. To account for the different types of degradation that can occur (i.e., demineralization, resin hydrolysis, and collagen degradation), enzymes such as esterase and collagenase found in the oral environment are used, in addition to acids, to form biochemical models to test resin-composite restorations in conjunction with mechanical loading. Furthermore, laboratory tests are usually performed in an accelerated manner to save time. It is argued that, for an accelerated multicomponent model to be representative and predictive in terms of both the mode and the speed of degradation, the individual components must be synchronized in their rates of action and be calibrated with clinical data. The process of calibrating the in vitro models against clinical data is briefly described. To achieve representative and predictive in vitro models, more comparative studies of in vivo and in vitro models are required to calibrate the laboratory studies.

Influence of the loci of non-cavitated fissure caries on its detection with optical coherence tomography.

OBJECTIVE: The main objective of this study was to evaluate the accuracy of optical coherence tomography (OCT) in detecting naturally occurring non-cavitated fissure caries (NCFC) in totality and at different loci by visually assessing cross-sectional OCT scans (B-scan) with an interpretation criterion. The secondary objective was to evaluate the agreement between dimensions of NCFC measured with OCT and polarized light microscopy (PLM). METHODS: 71 investigation sites of sound fissure and naturally occurring NCFC on human extracted premolars were identified and scanned with a swept-source OCT. The teeth were then sectioned bucco-lingually at the investigation sites and imaged using PLM. Two calibrated examiners trained on the B-scan NCFC visual interpretation criteria established for this study, assessed the investigation sites and results were validated against PLM. RESULTS: Detection sensitivity of B-scan for NCFC when fissures were assessed in totality, or on the slopes or walls separately are 0.98, 0.95, 0.94 and specificity are 0.95, 0.90, and 0.95. One-way ANOVA showed that width measurements of wall loci done with OCT and PLM were not statistically different. However, OCT height measurements of slope loci were statistically bigger with a constant bias of 0.08 mm (of which is not clinically significant) and OCT height measurements of wall loci were statistically smaller (0.57 mm) and Bland-Altman plots indicated presence of proportionate bias. CONCLUSION: Visual assessment of B-scans with the interpretation criteria resulted in both high specificity and sensitivity and were not affected by loci location. OCT width measurement of wall loci is in agreement with PLM. CLINICAL SIGNIFICANCE: Unanimous high sensitivity in this and previous studies indicate that visual assessment of B-scans reliably rule out NCFC. Detection accuracy was not affected by loci location. Width of wall loci and/or height of slope loci in OCT B-scan are to be used for monitoring NCFC but not height of wall loci.