Chemosensory gene expression in olfactory organs of the anthropophilic Anopheles coluzzii and zoophilic Anopheles quadriannulatus.

BACKGROUND: Anopheles (An.) coluzzii, one of Africa's primary malaria vectors, is highly anthropophilic. This human host preference contributes greatly to its ability to transmit malaria. In contrast, the closely related An. quadriannulatus prefers to feed on bovids and is not thought to contribute to malaria transmission. The diverged preference for host odor profiles between these sibling species is likely reflected in chemosensory gene expression levels in the olfactory organs. Therefore, we compared the transcriptomes of the antennae and maxillary palps between An. coluzzii and An. quadriannulatus, focusing on the major chemosensory gene families. RESULTS: While chemosensory gene expression is strongly correlated between the two species, various chemosensory genes show significantly enhanced expression in one of the species. In the antennae of An. coluzzii the expression of six olfactory receptors (Ors) and seven ionotropic receptors (Irs) is considerably enhanced, whereas 11 Ors and 3 Irs are upregulated in An. quadriannulatus. In the maxillary palps, leaving aside Irs with very low level of expression, one Ir is strongly enhanced in each species. In addition, we find divergence in odorant binding protein (Obp) gene expression, with several highly expressed Obps being enhanced in the antennae and palps of An. coluzzii. Finally, the expression of several gustatory receptors (Grs) in the palps appears to be species-specific, including a homolog of a sugar-sensing Drosophila Gr. CONCLUSIONS: A considerable number of Ors and Irs are differentially expressed between these two closely related species with diverging host preference. These chemosensory genes could play a role in the human host preference of the malaria vector An. coluzzii. Additionally, divergence in Obp expression between the two species suggests a possible role of these odor carrier proteins in determining host preference. Finally, divergence in chemosensory expression in the palps may point towards a possible role for the maxillary palps in host differentiation.

Stimulatory sublethal response of a generalist predator to permethrin: hormesis, hormoligosis, or homeostatic regulation?

The assessment of pesticide effects in arthropods historically have relied heavily on acute lethal effects. Although the sublethal responses to such compounds are sometimes neglected, stimulatory effects associated with low doses of compounds toxic at higher doses, such as pesticides, have been widely reported in recent years and recognized as a general toxicological phenomenon. Evidence of such stimulatory response has also been reported among mites and a few insect pest-species exposed to pesticides and recognized as a one of the potential causes underlying pest resurgence and secondary pest outbreaks. However, fitness parameters and its implications were seldom considered in these studies and natural enemies are not usually target of attention. Here, we reported the stimulatory effect of sublethal doses (ranging from 0.02 to 172.00 ppb in addition to the control) of the pyrethroid permethrin topically applied to third instar nymphs of the spined soldier bug, Podisus distinctus (Stål) (Heteroptera: Pentatomidae). The parameters estimated from the fertility tables of insects exposed to the increasing doses of insecticide indicated a slight increase in the mean survival time for doses > or = 0.20 ppb and a peak in the net reproductive rate at 1.72 ppb. This trend is coincident and correlated with the intrinsic rate of population growth (n = 18, r = 0.78, P = 0.0001), which also shows a peak at 1.72 ppb, leading to higher reproductive values of insects exposed to this dose. The phenomenon is consistent with insecticide-induced hormesis, for which the potential implications are discussed.